The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
PciI (3682) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
BstZ17I (3303) 1 site
GTATACCATATG
BsmI (3251) 1 site
GAATGCNCTTACGN
Sticky ends from different BsmI sites may not be compatible.
PfoI (3106) 1 site
TCCNGGAAGGNCCT
Sticky ends from different PfoI sites may not be compatible.
RsrII (2845) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BglII (12) 1 site
AGATCTTCTAGA
MfeI (161) 1 site
CAATTGGTTAAC
Bpu10I (180) 1 site
CCTNAGCGGANTCG
Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
NdeI (484) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.