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pcDNA™6/TR

Mammalian cell vector that yields high-level expression of the tetracycline repressor, for use with the T-REx™ system.

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pcDNA6 TR.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (6660) FspI (5961) AhdI (5739) PciI (4849) BspQI - SapI (4733) BstZ17I (4470) PfoI (4273) BlpI (4177) BsaBI * (4080) MscI (3785) SexAI * (3420) SpeI (249) Eco53kI (816) SacI (818) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (917) KpnI (921) BspEI * (1002) BspDI * - ClaI * (1008) KflI (1038) EcoNI (1892) AfeI (2088) AleI (2222) EcoRI (2323) BstXI (2491) NotI (2496) PaeR7I - PspXI - XhoI (2502) SacII (2521) BstBI (2525) AgeI (2575) pcDNA™6/TR 6662 bp
SgrDI  (6660)
1 site
C G T C G A C G G C A G C T G C
FspI  (5961)
1 site
T G C G C A A C G C G T
AhdI  (5739)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4849)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4733)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4733)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (4470)
1 site
G T A T A C C A T A T G
PfoI  (4273)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (4177)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsaBI  (4080)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MscI  (3785)
1 site
T G G C C A A C C G G T
SexAI  (3420)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SpeI  (249)
1 site
A C T A G T T G A T C A
Eco53kI  (816)
1 site
G A G C T C C T C G A G
SacI  (818)
1 site
G A G C T C C T C G A G
NheI  (895)
1 site
G C T A G C C G A T C G
BmtI  (899)
1 site
G C T A G C C G A T C G
AflII  (908)
1 site
C T T A A G G A A T T C
HindIII  (911)
1 site
A A G C T T T