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Plasmid Files

pcDNA™6/V5-His C

Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™6/V5‑His A or pcDNA™6/V5‑His B.

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pcDNA6 V5-His C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5138) SspI (5021) ScaI (4697) FspI (4439) AhdI (4217) PciI (3324) BspQI - SapI (3208) BstZ17I (2945) BsmI (2893) PfoI (2748) BlpI (2652) BglII (12) MfeI (161) Bpu10I (180) NdeI (484) SnaBI (590) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (917) KpnI (921) BamHI (929) EcoRI (952) PstI (961) EcoRV (964) NotI (979) PaeR7I - PspXI - XhoI (985) BstEII (990) BstBI (1000) AgeI (1050) SexAI * (1895) StuI (2127) AvrII (2128) TspMI - XmaI (2149) SmaI (2151) MscI (2260) EcoO109I - PpuMI (2416) BsgI (2444) BsaBI * (2555) pcDNA™6/V5-His C 5140 bp
SgrDI  (5138)
1 site
C G T C G A C G G C A G C T G C
SspI  (5021)
1 site
A A T A T T T T A T A A
ScaI  (4697)
1 site
A G T A C T T C A T G A
FspI  (4439)
1 site
T G C G C A A C G C G T
AhdI  (4217)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3324)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3208)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.