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Plasmid Files

phCMV1

Compact mammalian cell vector for high-level expression from an optimized CMV promoter.

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phCMV1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  MoBiTec
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AflIII - PciI (4181) ApaLI (3867) EcoO109I (3361) BsaI (3252) PfoI (3038) RsrII (2779) BsrDI (2496) PflFI - Tth111I (2381) FspI (2365) MscI (2345) PluTI (2266) SfoI (2264) NarI (2263) KasI (2262) NdeI (234) SnaBI (340) BsmBI (589) BbsI (634) BglII (833) PaeR7I - XhoI (837) HindIII (846) EcoRI (853) Acc65I (869) KpnI (873) PspOMI (877) TspMI - XmaI (880) ApaI (881) SmaI (882) BamHI (884) NotI (907) XbaI * (917) MfeI (1013) HpaI (1026) Bts α I (1102) AflII (1145) DraIII (1379) SexAI * (1852) SfiI (2038) BseRI (2081) StuI (2084) BspDI * - ClaI * (2103) phCMV1 4239 bp
AflIII  (4181)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4181)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3867)
1 site
G T G C A C C A C G T G
EcoO109I  (3361)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3252)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3038)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2779)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2496)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2381)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2381)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2365)
1 site
T G C G C A A C G C G T
MscI  (2345)
1 site
T G G C C A A C C G G T
PluTI  (2266)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2264)
1 site
G G C G C C C C G C G G
NarI  (2263)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2262)
1 site
G G C G C C C C G C G G
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
BsmBI  (589)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BbsI  (634)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (833)
1 site
A G A T C T T C T A G A
PaeR7I  (837)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (837)
1 site
C T C G A G G A G C T C
HindIII  (846)
1 site
A A G C T T T T C G A A
EcoRI  (853)
1 site
G A A T T C C T T A A G
Acc65I  (869)
1 site
G G T A C C C C A T G G
KpnI  (873)
1 site
G G T A C C C C A T G G
PspOMI  (877)
1 site
G G G C C C C C C G G G
TspMI  (880)
1 site
C C C G G G G G G C C C
XmaI  (880)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (881)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (882)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (884)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NotI  (907)
1 site
G C G G C C G C C G C C G G C G
XbaI  (917)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1013)
1 site
C A A T T G G T T A A C
HpaI  (1026)
1 site
G T T A A C C A A T T G
BtsαI  (1102)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AflII  (1145)
1 site
C T T A A G G A A T T C
DraIII  (1379)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (1852)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (2038)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (2081)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (2084)
1 site
A G G C C T T C C G G A
BspDI  (2103)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2103)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NeoR/KanR
2135 .. 2929  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2135 .. 2929  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
ori
3537 .. 4125  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication