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Plasmid Files

prHom-Nuc1

Mammalian vector encoding three FKBP homodimerizer domains, for creating nuclear oligomers that can be dissociated with a drug. Also known as pC4EN-FM3.

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prHom-Nuc1 Sequence and MapprHom-Nuc1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 PvuII (5952) NaeI (5750) NgoMIV (5748) DraIII (5647) PfoI (5232) XmnI (4798) BsaI (4248) AhdI (4187) BspQI - SapI (3178) SfiI (2993) MluI (1) SnaBI (298) EcoRI (656) ATG BmgBI (696) HA SV40 NLS XbaI (754) SpeI (1735) BamHI (1747) KflI (1776) HincII (1928) MfeI (2118) PspOMI (2293) ApaI (2297) Bsu36I (2397) MscI (2438) BstXI (2446) BglII (2467) PflMI (2673) AbsI - PaeR7I - PspXI - XhoI (2916) AvrII (2939) StuI (2944) prHom-Nuc1 6064 bp
PvuII  (5952)
1 site
C A G C T G G T C G A C
NaeI  (5750)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (5748)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
DraIII  (5647)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PfoI  (5232)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
XmnI  (4798)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (4248)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4187)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (3178)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3178)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
SfiI  (2993)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
MluI  (1)
1 site
A C G C G T T G C G C A
SnaBI  (298)
1 site
T A C G T A A T G C A T
EcoRI  (656)
1 site
G A A T T C C T T A A G
BmgBI  (696)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
XbaI  (754)
1 site
T C T A G A A G A T C T
SpeI  (1735)
1 site
A C T A G T T G A T C A
BamHI  (1747)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
KflI  (1776)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
HincII  (1928)
1 site
G T Y R A C C A R Y T G
MfeI  (2118)
1 site
C A A T T G G T T A A C
PspOMI  (2293)
1 site
G G G C C C C C C G G G
ApaI  (2297)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
Bsu36I  (2397)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
MscI  (2438)
1 site
T G G C C A A C C G G T
BstXI  (2446)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (2467)
1 site
A G A T C T T C T A G A
PflMI  (2673)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AbsI  (2916)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (2916)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2916)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2916)
1 site
C T C G A G G A G C T C
AvrII  (2939)
1 site
C C T A G G G G A T C C
StuI  (2944)
1 site
A G G C C T T C C G G A
AmpR
4114 .. 4986  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 2:  
   4114 .. 4917  =  804 bp
   267 amino acids  =  29.4 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4114 .. 4986  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 1:  signal sequence  
   4918 .. 4986  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4114 .. 4986  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
3355 .. 3943  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3355 .. 3943  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
β-globin intron
1766 .. 2338  =  573 bp
intron from rabbit β-globin gene
β-globin intron
1766 .. 2338  =  573 bp
intron from rabbit β-globin gene
f1 ori
5423 .. 5878  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
5423 .. 5878  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
DmrD
760 .. 1080  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
760 .. 1080  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1087 .. 1407  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1087 .. 1407  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1414 .. 1734  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1414 .. 1734  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 promoter
2923 .. 3118  =  196 bp
SV40 promoter
2923 .. 3118  =  196 bp
AmpR promoter
4987 .. 5091  =  105 bp
AmpR promoter
4987 .. 5091  =  105 bp
β-globin poly(A) signal
2535 .. 2590  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
2535 .. 2590  =  56 bp
rabbit β-globin polyadenylation signal
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus
thymidine kinase gene
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus
thymidine kinase gene
HA
685 .. 711  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin)
epitope tag
HA
685 .. 711  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin)
epitope tag
SV40 NLS
733 .. 753  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
733 .. 753  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
ATG
673 .. 675  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
673 .. 675  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
2943 .. 3078  =  136 bp
SV40 origin of replication
SV40 ori
2943 .. 3078  =  136 bp
SV40 origin of replication
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