Resources
Plasmid Files

pBin19

Binary Agrobacterium vector for plant transformation. Also known as pBIN19 or BIN19 or Bin19 or Bin 19.

 
To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pBin19.dna
Map and Sequence File:    Download    Open   
Download Free Trial Get SnapGene Viewer


EcoNI (10,149) PmeI (9285) Bsu36I (9074) BmtI (9062) NheI (9058) BstBI (8127) ApaI (7878) PspOMI (7874) AflII (7722) MauBI (7527) BspDI - ClaI (7238) XmnI (7118) HindIII (6823) SbfI (6815) SalI (6805) XbaI (6799) BamHI (6793) SmaI (6790) KpnI - TspMI - XmaI (6788) Acc65I (6784) SacI (6782) Eco53kI (6780) EcoRI (6772) DraIII (6471) AhdI (5806) NotI (92) PasI (645) PshAI (1412) MluI (1649) BstEII (1752) BstXI (2091) SfiI (3723) PflMI * (4052) NdeI (4281) AleI (4407) MfeI (4677) FspAI (5063) NruI (5560) KflI (5658) BbvCI (5748) pBin19 11,777 bp
EcoNI  (10,149)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PmeI  (9285)
1 site
G T T T A A A C C A A A T T T G
Bsu36I  (9074)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BmtI  (9062)
1 site
G C T A G C C G A T C G
NheI  (9058)
1 site
G C T A G C C G A T C G
BstBI  (8127)
1 site
T T C G A A A A G C T T
ApaI  (7878)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (7874)
1 site
G G G C C C C C C G G G
AflII  (7722)
1 site
C T T A A G G A A T T C
MauBI  (7527)
1 site
C G C G C G C G G C G C G C G C
BspDI  (7238)
1 site
A T C G A T T A G C T A
ClaI  (7238)
1 site
A T C G A T T A G C T A
XmnI  (7118)
1 site
G A A N N N N T T C C T T N N N N A A G
HindIII  (6823)
1 site
A A G C T T T T C G A A
SbfI  (6815)
1 site
C C T G C A G G G G A C G T C C
SalI  (6805)
1 site
G T C G A C C A G C T G
XbaI  (6799)
1 site
T C T A G A A G A T C T
BamHI  (6793)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (6790)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (6788)
1 site
G G T A C C C C A T G G
TspMI  (6788)
1 site
C C C G G G G G G C C C
XmaI  (6788)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (6784)
1 site
G G T A C C C C A T G G
SacI  (6782)
1 site
G A G C T C C T C G A G
Eco53kI  (6780)
1 site
G A G C T C C T C G A G
EcoRI  (6772)
1 site
G A A T T C C T T A A G
DraIII  (6471)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AhdI  (5806)
1 site
G A C N N N N N G T C C T G N N N