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Plasmid Files

pBin19

Binary Agrobacterium vector for plant transformation. Also known as pBIN19 or BIN19 or Bin19 or Bin 19.

 
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 EcoNI (10,149) PmeI (9285) Bsu36I (9074) BmtI (9062) NheI (9058) NeoR/KanR BstBI (8127) ApaI (7878) PspOMI (7874) AflII (7722) MauBI (7527) BspDI - ClaI (7238) XmnI (7118) lac operator HindIII (6823) SbfI (6815) SalI (6805) XbaI (6799) BamHI (6793) SmaI (6790) KpnI - TspMI - XmaI (6788) Acc65I (6784) SacI (6782) Eco53kI (6780) EcoRI (6772) DraIII (6471) AhdI (5806) NotI (92) PasI (645) PshAI (1412) MluI (1649) BstEII (1752) BstXI (2091) SfiI (3723) PflMI * (4052) NdeI (4281) AleI (4407) MfeI (4677) FspAI (5063) NruI (5560) KflI (5658) BbvCI (5748) pBin19 11,777 bp
EcoNI  (10,149)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PmeI  (9285)
1 site
G T T T A A A C C A A A T T T G
Bsu36I  (9074)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BmtI  (9062)
1 site
G C T A G C C G A T C G
NheI  (9058)
1 site
G C T A G C C G A T C G
BstBI  (8127)
1 site
T T C G A A A A G C T T
ApaI  (7878)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (7874)
1 site
G G G C C C C C C G G G
AflII  (7722)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
MauBI  (7527)
1 site
C G C G C G C G G C G C G C G C
BspDI  (7238)
1 site
A T C G A T T A G C T A
ClaI  (7238)
1 site
A T C G A T T A G C T A
XmnI  (7118)
1 site
G A A N N N N T T C C T T N N N N A A G
HindIII  (6823)
1 site
A A G C T T T T C G A A
SbfI  (6815)
1 site
C C T G C A G G G G A C G T C C
SalI  (6805)
1 site
G T C G A C C A G C T G
XbaI  (6799)
1 site
T C T A G A A G A T C T
BamHI  (6793)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (6790)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (6788)
1 site
G G T A C C C C A T G G
TspMI  (6788)
1 site
C C C G G G G G G C C C
XmaI  (6788)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (6784)
1 site
G G T A C C C C A T G G
SacI  (6782)
1 site
G A G C T C C T C G A G
Eco53kI  (6780)
1 site
G A G C T C C T C G A G
EcoRI  (6772)
1 site
G A A T T C C T T A A G
DraIII  (6471)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AhdI  (5806)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (92)
1 site
G C G G C C G C C G C C G G C G
PasI  (645)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PshAI  (1412)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MluI  (1649)
1 site
A C G C G T T G C G C A
BstEII  (1752)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (2091)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SfiI  (3723)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PflMI  (4052)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NdeI  (4281)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
AleI  (4407)
1 site
C A C N N N N G T G G T G N N N N C A C
MfeI  (4677)
1 site
C A A T T G G T T A A C
FspAI  (5063)
1 site
R T G C G C A Y Y A C G C G T R
NruI  (5560)
1 site
T C G C G A A G C G C T
KflI  (5658)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BbvCI  (5748)
1 site
C C T C A G C G G A G T C G
trfA
3369 .. 4517  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
trfA
3369 .. 4517  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
KanR
2276 .. 3070  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
2276 .. 3070  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
NeoR/KanR
8146 .. 8940  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
8146 .. 8940  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
IS1
1317 .. 2084  =  768 bp
prokaryotic transposable element
IS1
1317 .. 2084  =  768 bp
prokaryotic transposable element
oriV
11,760 .. 694  =  712 bp
incP origin of replication
oriV
11,760 .. 694  =  712 bp
incP origin of replication
TetR
9789 .. 10,439  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
9789 .. 10,439  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
traJ
11,262 .. 11,633  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
11,262 .. 11,633  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
lacZα
6522 .. 6845  =  324 bp
107 amino acids  =  12.1 kDa
Product: LacZα fragment of β-galactosidase
lacZα
6522 .. 6845  =  324 bp
107 amino acids  =  12.1 kDa
Product: LacZα fragment of β-galactosidase
NOS terminator
7504 .. 7756  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7504 .. 7756  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
8997 .. 9176  =  180 bp
nopaline synthase promoter
NOS promoter
8997 .. 9176  =  180 bp
nopaline synthase promoter
oriT
11,120 .. 11,229  =  110 bp
incP origin of transfer
oriT
11,120 .. 11,229  =  110 bp
incP origin of transfer
lac promoter
6889 .. 6919  =  31 bp
   Segment 3:  -10  
   6889 .. 6895  =  7 bp
promoter for the E. coli lac operon
lac promoter
6889 .. 6919  =  31 bp
   Segment 2:  
   6896 .. 6913  =  18 bp
promoter for the E. coli lac operon
lac promoter
6889 .. 6919  =  31 bp
   Segment 1:  -35  
   6914 .. 6919  =  6 bp
promoter for the E. coli lac operon
lac promoter
6889 .. 6919  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6113 .. 6137  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6113 .. 6137  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9300 .. 9324  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9300 .. 9324  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
6865 .. 6881  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
6865 .. 6881  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
6772 .. 6828  =  57 bp
pUC18/19 multiple cloning site
MCS
6772 .. 6828  =  57 bp
pUC18/19 multiple cloning site
M13 fwd
6755 .. 6771  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
6755 .. 6771  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
6841 .. 6857  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
6841 .. 6857  =  17 bp
common sequencing primer, one of multiple similar
variants
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