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Plasmid Files

pCAMBIA0380

Agrobacterium binary vector for plant transformation, with a kanamycin-resistance gene. The MCS is reversed in pCAMBIA0390.

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pCAMBIA0380 Sequence and MappCAMBIA0380.dna
Map and Sequence File   
Sequence Author:  Cambia
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 SpeI (6801) BglII (6794) NcoI (6787) HindIII (6768) PstI (6764) SalI (6754) BamHI (6748) SmaI (6745) TspMI - XmaI (6743) EcoRI (6738) AscI (6731) ApaI - BanII (6728) PspOMI (6724) BstXI (6721) AvrII (6708) XmnI (6703) AseI (6702) BclI * (6373) SacII (6370) PsiI (6242) Bpu10I (6097) EcoO109I - PpuMI (5958) BspHI (5916) BpmI (5915) BlpI (5766) EcoRV (5493) SspI (5420) BssS α I (4829) BstZ17I (4429) PluTI (4234) SfoI (4232) NarI (4231) KasI (4230) MreI - SgrAI (4227) AgeI (3939) BsaI (3848) EcoNI (3758) FspI (3662) BspDI * - ClaI * (3525) HpaI (6809) 6xHis DraIII - PmlI (23) BstEII (36) PvuI (60) AflII (87) MauBI (282) RB T-DNA repeat SphI (441) ScaI (472) PasI (1775) AclI (2369) BsiWI (3329) pCAMBIA0380 6813 bp
SpeI  (6801)
1 site
A C T A G T T G A T C A
BglII  (6794)
1 site
A G A T C T T C T A G A
NcoI  (6787)
1 site
C C A T G G G G T A C C
HindIII  (6768)
1 site
A A G C T T T T C G A A
PstI  (6764)
1 site
C T G C A G G A C G T C
SalI  (6754)
1 site
G T C G A C C A G C T G
BamHI  (6748)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (6745)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (6743)
1 site
C C C G G G G G G C C C
XmaI  (6743)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
EcoRI  (6738)
1 site
G A A T T C C T T A A G
AscI  (6731)
1 site
G G C G C G C C C C G C G C G G
ApaI  (6728)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (6728)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (6724)
1 site
G G G C C C C C C G G G
BstXI  (6721)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AvrII  (6708)
1 site
C C T A G G G G A T C C
XmnI  (6703)
1 site
G A A N N N N T T C C T T N N N N A A G
AseI  (6702)
1 site
A T T A A T T A A T T A
BclI  (6373)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SacII  (6370)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PsiI  (6242)
1 site
T T A T A A A A T A T T
Bpu10I  (6097)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (5958)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (5958)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspHI  (5916)
1 site
T C A T G A A G T A C T
BpmI  (5915)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BlpI  (5766)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoRV  (5493)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
SspI  (5420)
1 site
A A T A T T T T A T A A
BssSαI  (4829)
1 site
C A C G A G G T G C T C
BstZ17I  (4429)
1 site
G T A T A C C A T A T G
PluTI  (4234)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (4232)
1 site
G G C G C C C C G C G G
NarI  (4231)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (4230)
1 site
G G C G C C C C G C G G
MreI  (4227)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (4227)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AgeI  (3939)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsaI  (3848)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
EcoNI  (3758)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
FspI  (3662)
1 site
T G C G C A A C G C G T
BspDI  (3525)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3525)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
HpaI  (6809)
1 site
G T T A A C C A A T T G
DraIII  (23)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PmlI  (23)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BstEII  (36)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PvuI  (60)
1 site
C G A T C G G C T A G C
AflII  (87)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
MauBI  (282)
1 site
C G C G C G C G G C G C G C G C
SphI  (441)
1 site
G C A T G C C G T A C G
ScaI  (472)
1 site
A G T A C T T C A T G A
PasI  (1775)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
AclI  (2369)
1 site
A A C G T T T T G C A A
BsiWI  (3329)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
pVS1 RepA
2714 .. 3787  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
pVS1 RepA
2714 .. 3787  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
KanR
5392 .. 6186  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
5392 .. 6186  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
pVS1 StaA
1656 .. 2285  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
pVS1 StaA
1656 .. 2285  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
ori
4717 .. 5305  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4717 .. 5305  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
NOS terminator
58 .. 310  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
58 .. 310  =  253 bp
nopaline synthase terminator and poly(A) signal
pVS1 oriV
3853 .. 4047  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
pVS1 oriV
3853 .. 4047  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
bom
4391 .. 4531  =  141 bp
basis of mobility region from pBR322
bom
4391 .. 4531  =  141 bp
basis of mobility region from pBR322
MCS
6738 .. 6773  =  36 bp
multiple cloning site from pUC8
MCS
6738 .. 6773  =  36 bp
multiple cloning site from pUC8
RB T-DNA repeat
332 .. 356  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
332 .. 356  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6611 .. 6635  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6611 .. 6635  =  25 bp
left border repeat from nopaline C58 T-DNA
6xHis
6 .. 23  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
6 .. 23  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
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