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pCAMBIA1381

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance genes and GUS lacking a start codon.

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pCAMBIA1381.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Cambia
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HindIII (10,639) PstI (10,635) SalI (10,625) BamHI (10,619) EcoRI (10,609) AscI (10,602) ApaI - BanII (10,599) PspOMI (10,595) BstXI (10,592) AatII (9751) ZraI (9749) AsiSI (9412) RsrII (9363) PspXI (8711) SacII (8193) PsiI (8065) Bpu10I (7920) EcoO109I - PpuMI (7781) BlpI (7589) BstZ17I (6252) PluTI (6057) SfoI (6055) NarI (6054) KasI (6053) MreI - SgrAI (6050) BsaI (5671) EcoNI (5581) FspI (5485) BspDI * - ClaI * (5348) NcoI (0) BglII (7) SpeI (14) MfeI (147) SnaBI (401) PvuII (1090) BstBI (1110) PmlI (1849) BstEII (1859) AflII (1910) MauBI (2105) SphI (2264) PasI (3598) AclI (4192) BsiWI (5152) pCAMBIA1381 10,658 bp
HindIII  (10,639)
1 site
A A G C T T T T C G A A
PstI  (10,635)
1 site
C T G C A G G A C G T C
SalI  (10,625)
1 site
G T C G A C C A G C T G
BamHI  (10,619)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (10,609)
1 site
G A A T T C C T T A A G
AscI  (10,602)
1 site
G G C G C G C C C C G C G C G G
ApaI  (10,599)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (10,599)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (10,595)
1 site
G G G C C C C C C G G G
BstXI  (10,592)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AatII  (9751)
1 site
G A C G T C C T G C A G
ZraI  (9749)
1 site
G A C G T C C T G C A G
AsiSI  (9412)
1 site
G C G A T C G C C G C T A G C G
RsrII  (9363)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (8711)
1 site
V C T C G A G B B G A G C T C V
SacII  (8193)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PsiI  (8065)
1 site
T T A T A A A A T A T T
Bpu10I  (7920)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (7781)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (7781)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BlpI  (7589)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BstZ17I  (6252)
1 site
G T A T A C C A T A T G
PluTI  (6057)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (6055)
1 site
G G C G C C C C G C G G
NarI  (6054)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (6053)
1 site
G G C G C C C C G C G G
MreI  (6050)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (6050)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsaI  (5671)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
EcoNI  (5581)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
FspI  (5485)
1 site
T G C G C A A C G C G T
BspDI  (5348)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (5348)
1 site
A T C G A T T A G C T