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pCAMBIA2300

Agrobacterium binary vector for plant transformation, with kanamycin-resistance genes.

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pCAMBIA2300.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Cambia
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PmeI (8623) HindIII (8410) PstI - SbfI (8402) SalI (8392) XbaI (8386) BamHI (8380) SmaI (8377) KpnI - TspMI - XmaI (8375) Acc65I (8371) SacI (8369) Eco53kI (8367) EcoRI (8359) BstXI (8116) BglII (7316) NcoI (7301) BssHII (6771) RsrII (6653) PspXI (6451) BclI * (5936) SacII (5933) PsiI (5805) Bpu10I (5660) EcoO109I - PpuMI (5521) BspHI (5479) BlpI (5329) SspI (4983) NsiI (4945) BsaBI * (1331) PasI (1338) AclI (1932) BsiWI (2892) NheI (3008) BmtI (3012) EcoNI (3321) BsaI (3411) AgeI (3502) MreI - SgrAI (3790) BstZ17I (3992) pCAMBIA2300 8743 bp
PmeI  (8623)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8410)
1 site
A A G C T T T T C G A A
PstI  (8402)
1 site
C T G C A G G A C G T C
SbfI  (8402)
1 site
C C T G C A G G G G A C G T C C
SalI  (8392)
1 site
G T C G A C C A G C T G
XbaI  (8386)
1 site
T C T A G A A G A T C T
BamHI  (8380)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8377)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (8375)
1 site
G G T A C C C C A T G G
TspMI  (8375)
1 site
C C C G G G G G G C C C
XmaI  (8375)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (8371)
1 site
G G T A C C C C A T G G
SacI  (8369)
1 site
G A G C T C C T C G A G
Eco53kI  (8367)
1 site
G A G C T C C T C G A G
EcoRI  (8359)
1 site
G A A T T