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pGreenII 0179

Agrobacterium binary vector with kanamycin- and hygromycin-resistance genes.

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pGreenII 0179.dna
Map and Sequence File:    Download    Open   
Sequence Author:  pGreen website
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NruI (4821) PasI (4603) EcoNI (4565) SspI (4553) PflMI (4218) AlwNI (3691) BciVI (3478) BglII (3270) StuI (3217) BspQI - SapI (3093) SacI (2814) Eco53kI (2812) BstXI (2806) SacII (2805) AleI - MslI (2804) NotI (2793) XbaI (2786) SpeI (2780) BamHI (2774) SmaI (2770) TspMI - XmaI (2768) PstI (2766) EcoRI (2756) EcoRV (2752) HindIII (2744) AccI (2730) SalI (2729) AbsI - PaeR7I - PspXI - XhoI (2723) ApaI (2718) EcoO109I (2715) PspOMI (2714) KpnI (2712) Acc65I (2708) BsaBI * (62) StyI (421) NgoMIV (524) NaeI (526) FseI (528) AanI - PsiI (546) BglII (627) BlpI (919) BbvCI (1005) BtgZI (1397) NdeI (1621) RsrII (1662) BfuAI - BspMI (1756) ZraI (2048) AatII (2050) BmgBI (2211) FspI (2537) BmrI (2638) pGreenII 0179 5138 bp
NruI  (4821)
1 site
T C G C G A A G C G C T
PasI  (4603)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (4565)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (4553)
1 site
A A T A T T T T A T A A
PflMI  (4218)
1 site
C C