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pPZP122

Agrobacterium binary vector for plant transformation, with chloramphenicol- and gentamycin-resistance genes. The MCS is reversed in pPZP121.

 
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pPZP122.dna
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PmeI (8475) EcoRI (8196) BanII - SacI (8194) Eco53kI (8192) KpnI (8188) Acc65I (8184) SmaI (8182) TspMI - XmaI (8180) BamHI (8175) XbaI (8169) SalI (8163) PstI - SbfI (8161) HindIII (8145) BstXI (7968) NcoI (7155) PmlI (6522) BclI * (6056) BspEI (5552) BpmI (5432) SspI (5240) HpaI (5033) NsiI (4913) BsiWI (2860) NheI (2976) BmtI (2980) BspDI * - ClaI * (3056) EcoNI (3289) BsaI (3379) AgeI (3470) KasI (3761) NarI (3762) SfoI (3763) PluTI (3765) BstZ17I (3960) pPZP122 8627 bp
PmeI  (8475)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (8196)
1 site
G A A T T C C T T A A G
BanII  (8194)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (8194)
1 site
G A G C T C C T C G A G
Eco53kI  (8192)
1 site
G A G C T C C T C G A G
KpnI  (8188)
1 site
G G T A C C C C A T G G
Acc65I  (8184)
1 site
G G T A C C C C A T G G
SmaI  (8182)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (8180)
1 site
C C C G G G G G G C C C
XmaI  (8180)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BamHI  (8175)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (8169)
1 site
T C T A G A A G A T C T
SalI  (8163)
1 site
G T C G A C C A G C T G
PstI  (8161)
1 site
C T G C A G G A C G T C
SbfI  (8161)
1 site
C C T G C A G G G G A C G T C C
HindIII  (8145)
1 site
A A G C T T T T C G A A
BstXI  (7968)
1 site