Resources
Plasmid Files

pRI 101-AN

Agrobacterium binary vector for foreign gene expression in dicotyledonous plants.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pRI 101-AN.dna
Map and Sequence File:    Download    Open   
Sequence Author:  TaKaRa
Download Free Trial Get SnapGene Viewer


PmeI (9133) HindIII (9008) SphI (9006) PstI - SbfI (9000) BsaI (8913) ScaI (8783) PshAI (8340) XbaI (8143) NdeI (8080) SalI (8070) SmaI (8066) TspMI - XmaI (8064) KpnI (8062) Acc65I (8058) BamHI (8052) EcoRI (8046) SacI (7987) Eco53kI (7985) FspI (6982) BsaAI (6780) ApaI (6151) PspOMI (6147) PspXI (5493) BbvCI (5292) ApaLI (310) BstEII (1838) BstAPI (2018) PflMI (2086) BclI * (2394) AgeI (4300) AvrII (5073) pRI 101-AN 10,417 bp
PmeI  (9133)
1 site
G T T T A A A C C A A A T T T G
HindIII  (9008)
1 site
A A G C T T T T C G A A
SphI  (9006)
1 site
G C A T G C C G T A C G
PstI  (9000)
1 site
C T G C A G G A C G T C
SbfI  (9000)
1 site
C C T G C A G G G G A C G T C C
BsaI  (8913)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (8783)
1 site
A G T A C T T C A T G A
PshAI  (8340)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
XbaI  (8143)
1 site
T C T A G A A G A T C T
NdeI  (8080)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SalI  (8070)
1 site
G T C G A C C A G C T G
SmaI  (8066)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (8064)
1 site
C C C G G G G G G C C C
XmaI  (8064)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
KpnI  (8062)
1 site
G G T A C C C C A T G G
Acc65I  (8058)
1 site
G G T A C C C C A T G G
BamHI  (8052)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (8046)
1 site
G A A T T C C T T A A G
SacI  (7987)
1 site
G A G C T C C T C G A G
Eco53kI  (7985)
1 site
G A G C T C C T C G A G
FspI  (6982)
1 site
T G C G C A A C G C G T
BsaAI  (6780)
1 site
Y A C G T R R T G C A Y
ApaI  (6151)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (6147)
1 site
G G G C C C C C C G G G
PspXI  (5493)
1 site
V C T C G A G B B G A G C T C V
BbvCI  (5292)
1 site
C C T C A G C G G A G T C G
ApaLI  (310)
1 site
G T G C A C C A C G T G
BstEII  (1838)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstAPI  (2018)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PflMI  (2086)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BclI  (2394)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AgeI  (4300)
1 site
A C C G G T T G G C C A
AvrII  (5073)
1 site
C C T A G G G G A T C C
pRi replicator region
889 .. 5524  =  4636 bp
replicator region from Agrobacterium plasmid pRiA 4b
pRi replicator region
889 .. 5524  =  4636 bp
replicator region from Agrobacterium plasmid pRiA 4b
NeoR/KanR
6419 .. 7213  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6419 .. 7213  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
KanR
9256 .. 10,050  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
9256 .. 10,050  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
57 .. 645  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
57 .. 645  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CaMV 35S promoter
8157 .. 8502  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
8157 .. 8502  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
5893 .. 6145  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
5893 .. 6145  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7720 .. 7972  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7720 .. 7972  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
7270 .. 7449  =  180 bp
nopaline synthase promoter
NOS promoter
7270 .. 7449  =  180 bp
nopaline synthase promoter
MCS
7983 .. 8084  =  102 bp
multiple cloning site
MCS
7983 .. 8084  =  102 bp
multiple cloning site
AtADH 5'-UTR
8085 .. 8142  =  58 bp
translational enhancer from the 5'-UTR of the Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et al., 2008
AtADH 5'-UTR
8085 .. 8142  =  58 bp
translational enhancer from the 5'-UTR of the Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et al., 2008
lac promoter
7620 .. 7650  =  31 bp
   Segment 1:  -35  
   7620 .. 7625  =  6 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
   Segment 2:  
   7626 .. 7643  =  18 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
   Segment 3:  -10  
   7644 .. 7650  =  7 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
5531 .. 5555  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
5531 .. 5555  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9148 .. 9172  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9148 .. 9172  =  25 bp
right border repeat from nopaline C58 T-DNA
CAP binding site
7584 .. 7605  =  22 bp
CAP binding activates transcription in the presence of cAMP.
CAP binding site
7584 .. 7605  =  22 bp
CAP binding activates transcription in the presence of cAMP.
lac operator
7658 .. 7674  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
7658 .. 7674  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 rev
7682 .. 7698  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
7682 .. 7698  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  2422 .. 3444  =  1023 bp
ORF:  340 amino acids  =  37.6 kDa
ORF:  4879 .. 5142  =  264 bp
ORF:  87 amino acids  =  9.7 kDa
ORF:  1211 .. 2425  =  1215 bp
ORF:  404 amino acids  =  45.0 kDa
ORF:  6368 .. 6904  =  537 bp
ORF:  178 amino acids  =  19.9 kDa
ORF:  9389 .. 9619  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  3597 .. 4814  =  1218 bp
ORF:  405 amino acids  =  44.3 kDa
ORF:  5142 .. 5633  =  492 bp
ORF:  163 amino acids  =  18.0 kDa
ORF:  6075 .. 6368  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  10,410 .. 382  =  390 bp
ORF:  129 amino acids  =  14.4 kDa
ORF:  3467 .. 3742  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  4202 .. 4513  =  312 bp
ORF:  103 amino acids  =  10.9 kDa
ORF:  6419 .. 7213  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  8732 .. 8962  =  231 bp
ORF:  76 amino acids  =  9.1 kDa
ORF:  3148 .. 3387  =  240 bp
ORF:  79 amino acids  =  9.2 kDa
ORF:  3721 .. 4092  =  372 bp
ORF:  123 amino acids  =  13.9 kDa
ORF:  6655 .. 7041  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  9256 .. 10,050  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  6051 .. 6398  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  8256 .. 9005  =  750 bp
ORF:  249 amino acids  =  28.0 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2018 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter