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Plasmid Files

pQE-30

Bacterial vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE‑31 or pQE‑32.

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pQE-30.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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EcoO109I (3445) AatII (3391) ZraI (3389) XmnI (3068) PvuI (2839) FspI (2691) AseI (2641) NmeAIII (2617) BglI (2589) BsrFI (2549) BsaI (2530) AhdI (2469) AlwNI (1992) PspFI (1884) BseYI (1880) BbsI (3447) PaeR7I - XhoI (1) PsiI (49) MfeI (59) EcoRI (88) BseRI (115) BamHI (145) SphI (155) Eco53kI (159) BanII - SacI (161) Acc65I (163) KpnI (167) TspMI - XmaI (168) SmaI (170) SalI (173) HincII (175) PstI (183) BfuAI - BspMI (186) HindIII (187) BlpI (199) NheI (307) BmtI (311) Bpu10I (332) PvuII (459) BspEI (555) PasI - PflMI * (791) MscI (826) BtgI - NcoI - StyI (860) XbaI (1163) PfoI (1220) PflFI - Tth111I (1323) BsaAI (1330) BstZ17I (1349) NdeI (1399) BstAPI (1400) BspQI - SapI (1460) AflIII - PciI (1576) pQE-30 3461 bp
EcoO109I  (3445)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3391)
1 site
G A C G T C C T G C A G
ZraI  (3389)
1 site
G A C G T C C T G C A G
XmnI  (3068)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (2839)
1 site
C G A T C G G C T A G C
FspI  (2691)
1 site
T G C G C A A C G C G T
AseI  (2641)
1 site
A T T A A T T A A T T A
NmeAIII  (2617)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2589)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2549)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2530)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2469)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1992)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1884)
1 site
C C C A G C G G G T C G
BseYI  (1880)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (3447)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not b