Sticky ends from different BlpI sites may not be compatible.
SpeI (5427) 1 site
ACTAGTTGATCA
BamHI (5407) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BmtI (5390) 1 site
GCTAGCCGATCG
NheI (5386) 1 site
GCTAGCCGATCG
NdeI (5381) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NcoI (5315) 1 site
CCATGGGGTACC
XbaI (5276) 1 site
TCTAGAAGATCT
BglII (5210) 1 site
AGATCTTCTAGA
SgrAI (5169) 1 site
CRCCGGYGGYGGCCRC
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI (5021) 1 site
GCATGCCGTACG
EcoNI (4956) 1 site
CCTNNNNNAGGGGANNNNNTCC
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible.
MluI (4488) 1 site
ACGCGTTGCGCA
BclI (4474) 1 site
TGATCAACTAGT
* Blocked by Dam methylation.BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII (4306) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaI (4285) 1 site
GGGCCCCCCGGG
ApaI can be used between 25°C and 37°C.
PspOMI (4281) 1 site
GGGCCCCCCGGG
BssHII (4077) 1 site
GCGCGCCGCGCG
BssHII is typically used at 50°C, but is 75% active at 37°C.
HpaI (3986) 1 site
GTTAACCAATTG
PshAI (3647) 1 site
GACNNNNGTCCTGNNNNCAG
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EagI (3420) 1 site
CGGCCGGCCGGC
NruI (3389) 1 site
TCGCGAAGCGCT
BfuAI (3305) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI (3305) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmI (3006) 1 site
GAATGCNCTTACGN
Sticky ends from different BsmI sites may not be compatible.
BmeT110I (2935) 1 site
CYCGRGGRGCYC
AvaI (2934) 1 site
CYCGRGGRGCYC
Sticky ends from different AvaI sites may not be compatible.
BsoBI (2934) 1 site
CYCGRGGRGCYC
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.