Resources
Plasmid Files

pEU-His-FV

Flexi® Vector with the barnase gene, for in vitro transcription and translation of an N-terminally 6xHis-tagged protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pEU-His-FV.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Center for Eukaryotic Structural Genomics
Download Free Trial Get SnapGene Viewer


AfeI (24) PfoI (4692) FspI (3881) BglI (3779) BsaI (3720) AhdI (3659) AlwNI (3182) BglI (2411) SgrAI (106) NgoMIV (115) NaeI (117) XcmI (238) BsrGI (309) AsiSI - SgfI (549) Bpu10I (997) BspEI (1220) PasI - PflMI * (1456) PmeI (1675) Eco53kI (1688) SacI (1690) Acc65I (1692) KpnI (1696) XbaI (1707) SalI (1713) AccI (1714) HincII (1715) PstI - SbfI (1723) BlpI (1788) SpeI (1809) PshAI (1839) XcmI (2099) NsiI (2147) PmlI (2186) BsiWI - SnaBI (2193) BssHII (2250) HindIII (2392) pEU-His-FV 4966 bp
AfeI  (24)
1 site
A G C G C T T C G C G A
PfoI  (4692)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspI  (3881)
1 site
T G C G C A A C G C G T
BglI  (3779)
2 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3720)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3659)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3182)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BglI  (2411)
2 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SgrAI  (106)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
NgoMIV  (115)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (117)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
XcmI  (238)
2 sites
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (309)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AsiSI  (549)
1 site
G C G A T C G C C G C T A G C G
SgfI  (549)
1 site
G C G A T C G C C G C T A G C G
Bpu10I  (997)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (1220)
1 site
T C C G G A A G G C C T
PasI  (1456)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (1456)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
PmeI  (1675)
1 site
G T T T A A A C C A A A T T T G
Eco53kI  (1688)
1 site
G A G C T C C T C G A G
SacI  (1690)
1 site
G A G C T C C T C G A G
Acc65I  (1692)
1 site
G G T A C C C C A T G G
KpnI  (1696)
1 site
G G T A C C C C A T G G
XbaI  (1707)
1 site
T C T A G A A G A T C T
SalI  (1713)
1 site
G T C G A C C A G C T G
AccI  (1714)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (1715)
1 site
G T Y R A C C A R Y T G
PstI  (1723)
1 site
C T G C A G G A C G T C
SbfI  (1723)
1 site
C C T G C A G G G G A C G T C C
BlpI  (1788)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SpeI  (1809)
1 site
A C T A G T T G A T C A
PshAI  (1839)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
XcmI  (2099)
2 sites
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
NsiI  (2147)
1 site
A T G C A T T A C G T A
PmlI  (2186)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BsiWI  (2193)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (2193)
1 site
T A C G T A A T G C A T
BssHII  (2250)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HindIII  (2392)
1 site
A A G C T T T T C G A A
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3586 .. 4377  =  792 bp
   263 amino acids  =  29.0 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4378 .. 4446  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
CmR
1011 .. 1670  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1011 .. 1670  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
2827 .. 3415  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2827 .. 3415  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
576 .. 911  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
576 .. 911  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
AmpR promoter
4447 .. 4551  =  105 bp
AmpR promoter
4447 .. 4551  =  105 bp
lac UV5 promoter
927 .. 957  =  31 bp
   Segment 1:  -35  
   927 .. 932  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
927 .. 957  =  31 bp
   Segment 2:  
   933 .. 950  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
927 .. 957  =  31 bp
   Segment 3:  -10  
   951 .. 957  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
927 .. 957  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
SP6 promoter
373 .. 391  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
373 .. 391  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
6xHis
527 .. 544  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
527 .. 544  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ATG
521 .. 523  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
521 .. 523  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ORF:  3716 .. 3982  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4922 .. 267  =  312 bp
ORF:  103 amino acids  =  10.6 kDa
ORF:  576 .. 911  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  1011 .. 1670  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  4802 .. 183  =  348 bp
ORF:  115 amino acids  =  11.9 kDa
ORF:  3586 .. 4446  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2018 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter