Resources
Plasmid Files

pVP33K

Bacterial Flexi® Vector with a kanamycin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-tetracysteine-TEV cassette to an expressed protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pVP33K.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Center for Eukaryotic Structural Genomics
Download Free Trial Get SnapGene Viewer


PaeR7I - XhoI (117) EagI (7040) NaeI (6448) NgoMIV (6446) RsrII (6431) AvrII (6279) PciI (5459) NdeI (5282) BstZ17I (5232) FspAI (5039) PpuMI * (5022) BstEII (4387) EcoRV (4123) HpaI (4067) AanI (165) MfeI (175) BseRI (231) NsiI (263) BsaI (327) AanI (616) BmgBI (806) AfeI (1174) PacI (1369) AsiSI - SgfI (1438) PmeI (2564) Eco53kI (2577) SacI (2579) SbfI (2612) BsrGI (2721) AscI (2738) SgrDI (2753) BspDI - ClaI (2766) BstBI (2777) HindIII (2780) NheI (2900) BmtI (2904)