Resources
Plasmid Files

pETBlue™-1 AccepTor™

Linearized vector with 3'-U overhangs for UA cloning and inducible bacterial expression of PCR-amplified genes.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pETBlue-1 AccepTor Sequence and MappETBlue-1 AccepTor.dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 AgeI (3145) NspI (2813) AflIII - PciI (2809) PspFI (2509) BseYI (2505) AlwNI (2400) BspHI (2089) XmnI (1884) ScaI (1765) TatI (1763) NmeAIII (1433) T7 promoter XbaI (3246) BglII (3422) NheI (3434) BmtI (3438) End (3478) Start (1) EcoRI (5) AvaI - BsoBI - TspMI - XmaI (14) BmeT110I (15) SmaI - SrfI (16) tet promoter AvrII (143) BlpI (547) BspEI (624) BanII (801) BtgZI (867) BsaAI (872) DraIII (875) PsiI (1000) SspI (1080) AhdI (1285) pETBlue™-1 AccepTor™ 3477 bp
AgeI  (3145)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
NspI  (2813)
1 site
R C A T G Y Y G T A C R
AflIII  (2809)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2809)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (2509)
1 site
C C C A G C G G G T C G
BseYI  (2505)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AlwNI  (2400)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspHI  (2089)
1 site
T C A T G A A G T A C T
XmnI  (1884)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (1765)
1 site
A G T A C T T C A T G A
TatI  (1763)
1 site
W G T A C W W C A T G W
NmeAIII  (1433)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XbaI  (3246)
1 site
T C T A G A A G A T C T
BglII  (3422)
1 site
A G A T C T T C T A G A
NheI  (3434)
1 site
G C T A G C C G A T C G
BmtI  (3438)
1 site
G C T A G C C G A T C G
End  (3478)
0 sites
Start  (1)
0 sites
EcoRI  (5)
1 site
G A A T T C C T T A A G
AvaI  (14)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (14)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
TspMI  (14)
1 site
C C C G G G G G G C C C
XmaI  (14)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (15)
1 site
C Y C G R G G R G C Y C
SmaI  (16)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (16)
1 site
G C C C G G G C C G G G C C C G
AvrII  (143)
1 site
C C T A G G G G A T C C
BlpI  (547)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BspEI  (624)
1 site
T C C G G A A G G C C T
BanII  (801)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BtgZI  (867)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (872)
1 site
Y A C G T R R T G C A Y
DraIII  (875)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (1000)
1 site
T T A T A A A A T A T T
SspI  (1080)
1 site
A A T A T T T T A T A A
AhdI  (1285)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AmpR
1212 .. 2072  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1212 .. 2003  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1212 .. 2072  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2004 .. 2072  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1212 .. 2072  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2165 .. 2753  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2165 .. 2753  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
642 .. 1097  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
642 .. 1097  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
lacZα
3253 .. 3477  =  225 bp
74 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
3253 .. 3477  =  225 bp
74 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
rrnB T1 terminator
285 .. 371  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
285 .. 371  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
T7 terminator
558 .. 605  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
558 .. 605  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
lacZα
2 .. 37  =  36 bp
12 amino acids  =  1.4 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2 .. 37  =  36 bp
12 amino acids  =  1.4 kDa
Product: LacZα fragment of β-galactosidase
tet promoter
87 .. 115  =  29 bp
   Segment 3:  -10  
   87 .. 92  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
87 .. 115  =  29 bp
   Segment 2:  
   93 .. 109  =  17 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
87 .. 115  =  29 bp
   Segment 1:  -35  
   110 .. 115  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
87 .. 115  =  29 bp
3 segments
E. coli promoter for tetracycline efflux protein gene
rrnB T2 terminator
463 .. 490  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
rrnB T2 terminator
463 .. 490  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
lac operator
3219 .. 3243  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
3219 .. 3243  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator (symmetric)
3152 .. 3171  =  20 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG). The
symmetric lac operator was optimized for tight
binding of lac repressor.
lac operator (symmetric)
3152 .. 3171  =  20 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG). The
symmetric lac operator was optimized for tight
binding of lac repressor.
T7 promoter
3200 .. 3218  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3200 .. 3218  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
3464 .. 3469  =  6 bp
ribosome binding site
RBS
3464 .. 3469  =  6 bp
ribosome binding site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter