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Plasmid Files

pAd/BLOCK-iT™-DEST

Adenoviral Gateway® destination vector for inserting an shRNA cassette from a U6 entry vector.

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pAd BLOCK-iT-DEST Sequence and MappAd BLOCK-iT-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
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 5' ITR PacI (34,861) PacI (32,787) BstBI (29,683) SpeI (25,803) MreI (25,110) AsiSI (20,163) attR1 lac UV5 promoter attR2 PmeI (11,979) pAd/BLOCK-iT™-DEST 34,864 bp
PacI  (34,861)
2 sites
T T A A T T A A A A T T A A T T
PacI  (32,787)
2 sites
T T A A T T A A A A T T A A T T
BstBI  (29,683)
1 site
T T C G A A A A G C T T
SpeI  (25,803)
1 site
A C T A G T T G A T C A
MreI  (25,110)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (20,163)
1 site
G C G A T C G C C G C T A G C G
PmeI  (11,979)
1 site
G T T T A A A C C A A A T T T G
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5,
lacking the E1 and E3 regions
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5,
lacking the E1 and E3 regions
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 2:  
   33,746 .. 34,537  =  792 bp
   263 amino acids  =  28.8 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 1:  signal sequence  
   34,538 .. 34,606  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
34,607 .. 34,711  =  105 bp
AmpR promoter
34,607 .. 34,711  =  105 bp
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 1:  -35  
   661 .. 666  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 2:  
   667 .. 684  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 3:  -10  
   685 .. 691  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
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