Resources
Plasmid Files

pAd/PL-DEST™

Promoterless adenoviral Gateway® destination vector for inserting a gene expression cassette.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pAd PL-DEST.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer


BstBI (29,683) SpeI (25,803) MreI (25,110) AsiSI (20,163) PmeI (11,979) pAd/PL-DEST™ 34,864 bp
BstBI  (29,683)
1 site
T T C G A A A A G C T T
SpeI  (25,803)
1 site
A C T A G T T G A T C A
MreI  (25,110)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (20,163)
1 site
G C G A T C G C C G C T A G C G
PmeI  (11,979)
1 site
G T T T A A A C C A A A T T T G
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 2:  
   33,746 .. 34,537  =  792 bp
   263 amino acids  =  28.8 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
   Segment 1:  signal sequence  
   34,538 .. 34,606  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  31.4 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
34,607 .. 34,711  =  105 bp
AmpR promoter
34,607 .. 34,711  =  105 bp
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 1:  -35  
   661 .. 666  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 2:  
   667 .. 684  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
   Segment 3:  -10  
   685 .. 691  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ORF:  745 .. 1404  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5011 .. 5331  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  6031 .. 6282  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  7216 .. 7452  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  12,877 .. 14,592  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  14,599 .. 15,195  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  15,232 .. 16,371  =  1140 bp
ORF:  379 amino acids  =  42.6 kDa
ORF:  16,399 .. 16,641  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  17,563 .. 20,421  =  2859 bp
ORF:  952 amino acids  =  108.0 kDa
ORF:  22,456 .. 22,812  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  23,155 .. 23,541  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  24,718 .. 25,506  =  789 bp
ORF:  262 amino acids  =  28.9 kDa
ORF:  29,578 .. 29,976  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  30,799 .. 31,296  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  32,851 .. 33,087  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  2330 .. 2752  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  9152 .. 9565  =  414 bp
ORF:  137 amino acids  =  14.4 kDa
ORF:  11,039 .. 12,796  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  16,007 .. 16,486  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  16,724 .. 17,476  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  25,895 .. 26,578  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  26,579 .. 26,902  =  324 bp
ORF:  107 amino acids  =  12.3 kDa
ORF:  27,320 .. 27,682  =  363 bp
ORF:  120 amino acids  =  13.7 kDa
ORF:  31,310 .. 31,648  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  32,933 .. 33,331  =  399 bp
ORF:  132 amino acids  =  14.8 kDa
ORF:  1746 .. 2051  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  6699 .. 7148  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  8025 .. 8531  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  9771 .. 11,018  =  1248 bp
ORF:  415 amino acids  =  47.1 kDa
ORF:  20,454 .. 21,068  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  22,200 .. 25,205  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  27,828 .. 29,630  =  1803 bp
ORF:  600 amino acids  =  63.6 kDa
ORF:  33,876 .. 34,142  =  267 bp
ORF:  88 amino acids  =  9.1 kDa
ORF:  4058 .. 4405  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  7304 .. 9265  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  10,280 .. 10,573  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  17,186 .. 17,515  =  330 bp
ORF:  109 amino acids  =  11.4 kDa
ORF:  21,164 .. 22,753  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  25,148 .. 25,438  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  28,001 .. 28,471  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  28,805 .. 29,050  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  31,196 .. 31,546  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  33,746 .. 34,606  =  861 bp
ORF:  286 amino acids  =  31.4 kDa
ORF:  2812 .. 4170  =  1359 bp
ORF:  452 amino acids  =  51.2 kDa
ORF:  5173 .. 5511  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  7183 .. 7761  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  9475 .. 9840  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  11,035 .. 11,295  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  13,102 .. 13,887  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  15,364 .. 15,633  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  16,882 .. 17,430  =  549 bp
ORF:  182 amino acids  =  18.9 kDa
ORF:  30,841 .. 31,185  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  31,984 .. 32,370  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  32,740 .. 33,036  =  297 bp
ORF:  98 amino acids  =  10.8 kDa
ORF:  3195 .. 3419  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  3918 .. 7088  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  9981 .. 10,349  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  15,918 .. 16,169  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  16,368 .. 16,658  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  17,595 .. 17,870  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  30,036 .. 30,920  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2018 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter