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Plasmid Files

pAdenoX-PRLS-DsRed-Express Linear

In-Fusion®-ready linearized promoterless adenoviral vector for inserting a gene cassette and co-expressing with DsRed-Express.

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pAdenoX-PRLS-DsRed-Express Linear.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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End (34,313) Adeno-X fwd screening primer (34,051 .. 34,075) AsuII - Bpu14I - Bsp119I - BspT104I - BstBI - Csp45I - NspV - SfuI (30,980) I-PpoI (24,385) SrfI (24,036) AhlI - BcuI - SpeI (23,588) MreI (22,901) BamHI (18,074) Start (0) Adeno-X rev screening primer (19 .. 38) BssNAI - Bst1107I - BstZ17I (2280) MssI - PmeI (9770) AsiSI - RgaI - SfaAI - SgfI (17,954) pAdenoX-PRLS-DsRed-Express Linear 34,313 bp
End  (34,313)
0 sites
AsuII  (30,980)
1 site
T T C G A A A A G C T T
Bpu14I  (30,980)
1 site
T T C G A A A A G C T T
Bsp119I  (30,980)
1 site
T T C G A A A A G C T T
BspT104I  (30,980)
1 site
T T C G A A A A G C T T
BstBI  (30,980)
1 site
T T C G A A A A G C T T
Csp45I  (30,980)
1 site
T T C G A A A A G C T T
NspV  (30,980)
1 site
T T C G A A A A G C T T
SfuI  (30,980)
1 site
T T C G A A A A G C T T
I-PpoI  (24,385)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
SrfI  (24,036)
1 site
G C C C G G G C C G G G C C C G
AhlI  (23,588)
1 site
A C T A G T T G A T C A
BcuI  (23,588)
1 site
A C T A G T T G A T C A
SpeI  (23,588)
1 site
A C T A G T T G A T C A
MreI  (22,901)
1 site
C G C C G G C G G C G G C C G C
BamHI  (18,074)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
BssNAI  (2280)
1 site
G T A T A C C A T A T G
Bst1107I  (2280)
1 site
G T A T A C C A T A T G
BstZ17I  (2280)
1 site
G T A T A C C A T A T G
MssI  (9770)
1 site
G T T T A A A C C A A A T T T G
PmeI  (9770)
1 site
G T T T A A A C C A A A T T T G
AsiSI  (17,954)
1 site
G C G A T C G C C G C T A G C G
RgaI  (17,954)
1 site
G C G A T C G C C G C T A G C G
SfaAI  (17,954)
1 site
G C G A T C G C C G C T A G C G
SgfI  (17,954)
1 site
G C G A T C G C C G C T A G C G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
34,051 .. 34,075  =  25 annealed bases
Tm  =  63°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
19 .. 38  =  20 annealed bases
Tm  =  57°C
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
26,020 .. 30,855  =  4836 bp
adenovirus DNA
26,020 .. 30,855  =  4836 bp
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   31,859 .. 31,927  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   31,928 .. 32,719  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
DsRed-Express
25,063 .. 25,740  =  678 bp
225 amino acids  =  25.7 kDa
DsRed-Express
25,063 .. 25,740  =  678 bp
225 amino acids  =  25.7 kDa
ori
32,890 .. 33,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
32,890 .. 33,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
31,220 .. 31,675  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
31,220 .. 31,675  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
Ad5 Ψ
34,138 .. 34,288  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
34,138 .. 34,288  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
31,754 .. 31,858  =  105 bp
AmpR promoter
31,754 .. 31,858  =  105 bp
ITR
30,856 .. 30,958  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
30,856 .. 30,958  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
33,948 .. 34,050  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
33,948 .. 34,050  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
SV40 poly(A) signal
25,861 .. 25,942  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
25,861 .. 25,942  =  82 bp
SV40 polyadenylation signal
M13 fwd
31,063 .. 31,079  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
31,063 .. 31,079  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
33,864 .. 33,880  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
33,864 .. 33,880  =  17 bp
common sequencing primer, one of multiple similar variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
34,299 .. 34,313  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
34,299 .. 34,313  =  15 bp
sequence to be added to 5' end of forward primer
ORF:  4492 .. 4941  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  5818 .. 6324  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  8830 .. 10,587  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  10,669 .. 12,384  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  12,391 .. 12,987  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  13,024 .. 14,163  =  1140 bp
ORF:  379 amino acids  =  42.7 kDa
ORF:  14,191 .. 14,433  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  23,680 .. 24,363  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  25,063 .. 25,740  =  678 bp
ORF:  225 amino acids  =  25.7 kDa
ORF:  26,050 .. 27,807  =  1758 bp
ORF:  585 amino acids  =  62.1 kDa
ORF:  2804 .. 3124  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  3824 .. 4075  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  5009 .. 5245  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  7562 .. 8809  =  1248 bp
ORF:  415 amino acids  =  47.0 kDa
ORF:  10,496 .. 10,726  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  13,799 .. 14,278  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  14,516 .. 15,268  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  18,245 .. 18,859  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  19,991 .. 22,996  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  27,755 .. 28,153  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  28,976 .. 29,473  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  31,859 .. 32,719  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  123 .. 545  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  6945 .. 7325  =  381 bp
ORF:  126 amino acids  =  13.5 kDa
ORF:  15,354 .. 18,212  =  2859 bp
ORF:  952 amino acids  =  108.0 kDa
ORF:  20,247 .. 20,603  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  20,946 .. 21,332  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  22,509 .. 23,291  =  783 bp
ORF:  260 amino acids  =  28.8 kDa
ORF:  29,487 .. 29,825  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  1851 .. 2198  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  5097 .. 7058  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  7266 .. 7631  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  8826 .. 9086  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  13,710 .. 13,961  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  14,160 .. 14,450  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  24,882 .. 25,115  =  234 bp
ORF:  77 amino acids  =  8.2 kDa
ORF:  26,178 .. 26,648  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  26,982 .. 27,227  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  29,373 .. 29,723  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  31,458 .. 31,703  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  605 .. 1963  =  1359 bp
ORF:  452 amino acids  =  51.1 kDa
ORF:  2966 .. 3304  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  4976 .. 5554  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  7772 .. 8140  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  15,386 .. 15,661  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  29,018 .. 29,362  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  29,720 .. 30,130  =  411 bp
ORF:  136 amino acids  =  15.3 kDa
ORF:  988 .. 1212  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  1711 .. 4881  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  6814 .. 7227  =  414 bp
ORF:  137 amino acids  =  15.3 kDa
ORF:  8071 .. 8364  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  10,375 .. 10,632  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  10,894 .. 11,679  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  13,156 .. 13,425  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  14,674 .. 15,306  =  633 bp
ORF:  210 amino acids  =  22.5 kDa
ORF:  18,955 .. 20,544  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  22,939 .. 23,229  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  25,042 .. 25,770  =  729 bp
ORF:  242 amino acids  =  24.3 kDa
ORF:  28,213 .. 29,097  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
ORF:  30,160 .. 30,546  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  32,323 .. 32,589  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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