pAdenoX-PRLS Linear

In-Fusion®-ready linearized promoterless adenoviral vector for inserting an entire gene expression cassette.

Sequence Author: Clontech (TaKaRa)

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End (32,665) In-Fusion Cloning site Adeno-X fwd screening primer (32,403 .. 32,427) ITR M13 rev M13 fwd XbaI (29,360) BstBI (29,332) SrfI (24,036) SpeI (23,588) MreI (22,901) BamHI (18,074) Start (0) Adeno-X rev screening primer (19 .. 38) BstZ17I (2280) PmeI (9770) AsiSI (17,954) pAdenoX-PRLS Linear 32,665 bp
End  (32,665)
0 sites
XbaI  (29,360)
1 site
T C T A G A A G A T C T
BstBI  (29,332)
1 site
T T C G A A A A G C T T
SrfI  (24,036)
1 site
G C C C G G G C C G G G C C C G
SpeI  (23,588)
1 site
A C T A G T T G A T C A
MreI  (22,901)
1 site
C G C C G G C G G C G G C C G C
BamHI  (18,074)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
BstZ17I  (2280)
1 site
G T A T A C C A T A T G
PmeI  (9770)
1 site
G T T T A A A C C A A A T T T G
AsiSI  (17,954)
1 site
G C G A T C G C C G C T A G C G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
32,403 .. 32,427  =  25 annealed bases
Tm  =  64°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
19 .. 38  =  20 annealed bases
Tm  =  56°C
adenovirus DNA
39 .. 29,207  =  29,169 bp
adenovirus DNA
39 .. 29,207  =  29,169 bp
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   30,211 .. 30,279  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   30,280 .. 31,071  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
31,242 .. 31,830  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
31,242 .. 31,830  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
29,572 .. 30,027  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
29,572 .. 30,027  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
Ad5 Ψ
32,490 .. 32,640  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
32,490 .. 32,640  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
30,106 .. 30,210  =  105 bp
AmpR promoter
30,106 .. 30,210  =  105 bp
ITR
29,208 .. 29,310  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
29,208 .. 29,310  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
32,300 .. 32,402  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
32,300 .. 32,402  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
M13 fwd
29,415 .. 29,431  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
29,415 .. 29,431  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
32,216 .. 32,232  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
32,216 .. 32,232  =  17 bp
common sequencing primer, one of multiple similar variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
32,651 .. 32,665  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
32,651 .. 32,665  =  15 bp
sequence to be added to 5' end of forward primer
ORF:  4492 .. 4941  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  5818 .. 6324  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  8830 .. 10,587  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  10,669 .. 12,384  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  12,391 .. 12,987  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  13,024 .. 14,163  =  1140 bp
ORF:  379 amino acids  =  42.7 kDa
ORF:  14,191 .. 14,433  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  23,680 .. 24,363  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  26,107 .. 26,505  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  27,328 .. 27,825  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  30,211 .. 31,071  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2804 .. 3124  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  3824 .. 4075  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  5009 .. 5245  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  7562 .. 8809  =  1248 bp
ORF:  415 amino acids  =  47.0 kDa
ORF:  10,496 .. 10,726  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  13,799 .. 14,278  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  14,516 .. 15,268  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  18,245 .. 18,859  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  19,991 .. 22,996  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  27,839 .. 28,177  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  123 .. 545  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  6945 .. 7325  =  381 bp
ORF:  126 amino acids  =  13.5 kDa
ORF:  15,354 .. 18,212  =  2859 bp
ORF:  952 amino acids  =  107.9 kDa
ORF:  20,247 .. 20,603  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  20,946 .. 21,332  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  22,509 .. 23,291  =  783 bp
ORF:  260 amino acids  =  28.8 kDa
ORF:  24,402 .. 26,159  =  1758 bp
ORF:  585 amino acids  =  62.1 kDa
ORF:  605 .. 1963  =  1359 bp
ORF:  452 amino acids  =  51.1 kDa
ORF:  2966 .. 3304  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  4976 .. 5554  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  7772 .. 8140  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  15,386 .. 15,661  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  24,530 .. 25,000  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  25,334 .. 25,579  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  27,725 .. 28,075  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  29,810 .. 30,055  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  988 .. 1212  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  1711 .. 4881  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  6814 .. 7227  =  414 bp
ORF:  137 amino acids  =  15.3 kDa
ORF:  8071 .. 8364  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  10,375 .. 10,632  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  10,894 .. 11,679  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  13,156 .. 13,425  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  14,674 .. 15,306  =  633 bp
ORF:  210 amino acids  =  22.5 kDa
ORF:  18,955 .. 20,544  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  22,939 .. 23,229  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  27,370 .. 27,714  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  28,072 .. 28,482  =  411 bp
ORF:  136 amino acids  =  15.3 kDa
ORF:  1851 .. 2198  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  5097 .. 7058  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  7266 .. 7631  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  8826 .. 9086  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  13,710 .. 13,961  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  14,160 .. 14,450  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  26,565 .. 27,449  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
ORF:  28,512 .. 28,898  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  30,675 .. 30,941  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pAdenoX-PRLS Linear.dna file

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