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Plasmid Files

pCMV-VSV-G

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

 
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 XbaI (6502) NotI (6495) SacII (6489) AleI (6488) BssHII (6444) NaeI (6155) NgoMIV (6153) XmnI (5509) NmeAIII (5058) AhdI (4910) PspFI (4325) BseYI (4321) BspQI - SapI (3901) BglII (3195) BsrGI (41) SpeI (97) SnaBI (438) BsmBI (687) SalI (753) AccI (754) PpuMI - SanDI (788) MfeI (1130) PspOMI (1305) ApaI (1309) BamHI (1411) XhoI (1417) SwaI (1572) PasI (2074) EcoNI (2078) XcmI (2184) PstI (2224) AgeI (2417) Acc65I (2444) KpnI (2448) BclI * (2520) BmgBI (2578) Bpu10I (2688) BstBI (2697) XhoI (3097) BamHI (3103) Bsu36I (3125) MscI (3166) pCMV-VSV-G 6507 bp
XbaI  (6502)
1 site
T C T A G A A G A T C T
NotI  (6495)
1 site
G C G G C C G C C G C C G G C G
SacII  (6489)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
AleI  (6488)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (6444)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NaeI  (6155)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (6153)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
XmnI  (5509)
1 site
G A A N N N N T T C C T T N N N N A A G
NmeAIII  (5058)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (4910)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (4325)
1 site
C C C A G C G G G T C G
BseYI  (4321)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BspQI  (3901)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3901)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BglII  (3195)
1 site
A G A T C T T C T A G A
BsrGI  (41)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (97)
1 site
A C T A G T T G A T C A
SnaBI  (438)
1 site
T A C G T A A T G C A T
BsmBI  (687)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SalI  (753)
1 site
G T C G A C C A G C T G
AccI  (754)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PpuMI  (788)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (788)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
MfeI  (1130)
1 site
C A A T T G G T T A A C
PspOMI  (1305)
1 site
G G G C C C C C C G G G
ApaI  (1309)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (1411)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XhoI  (1417)
2 sites
C T C G A G G A G C T C
SwaI  (1572)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PasI  (2074)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (2078)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XcmI  (2184)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PstI  (2224)
1 site
C T G C A G G A C G T C
AgeI  (2417)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
Acc65I  (2444)
1 site
G G T A C C C C A T G G
KpnI  (2448)
1 site
G G T A C C C C A T G G
BclI  (2520)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BmgBI  (2578)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
Bpu10I  (2688)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (2697)
1 site
T T C G A A A A G C T T
XhoI  (3097)
2 sites
C T C G A G G A G C T C
BamHI  (3103)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Bsu36I  (3125)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
MscI  (3166)
1 site
T G G C C A A C C G G T
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
   Segment 1:  signal peptide  
   1436 .. 1483  =  48 bp
   16 amino acids  =  1.8 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
   Segment 2:  
   1484 .. 2971  =  1488 bp
   495 amino acids  =  55.7 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4837 .. 5628  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5629 .. 5697  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
AmpR promoter
5698 .. 5802  =  105 bp
AmpR promoter
5698 .. 5802  =  105 bp
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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