pCMV-VSV-G

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.
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XbaI (6502) NotI (6495) SacII (6489) AleI (6488) BssHII (6444) NaeI (6155) NgoMIV (6153) XmnI (5509) NmeAIII (5058) AhdI (4910) PspFI (4325) BseYI (4321) BspQI - SapI (3901) BglII (3195) BsrGI (41) SpeI (97) SnaBI (438) BsmBI (687) SalI (753) AccI (754) PpuMI - SanDI (788) MfeI (1130) PspOMI (1305) ApaI (1309) BamHI (1411) XhoI (1417) SwaI (1572) PasI (2074) EcoNI (2078) XcmI (2184) PstI (2224) AgeI (2417) Acc65I (2444) KpnI (2448) BclI * (2520) BmgBI (2578) Bpu10I (2688) BstBI (2697) XhoI (3097) BamHI (3103) Bsu36I (3125) MscI (3166) pCMV-VSV-G 6507 bp
XbaI  (6502)
1 site
T C T A G A A G A T C T
NotI  (6495)
1 site
G C G G C C G C C G C C G G C G
SacII  (6489)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (6488)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (6444)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NaeI  (6155)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6153)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
XmnI  (5509)
1 site
G A A N N N N T T C C T T N N N N A A G
NmeAIII  (5058)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (4910)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (4325)
1 site
C C C A G C G G G T C G
BseYI  (4321)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (3901)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3901)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BglII  (3195)
1 site
A G A T C T T C T A G A
BsrGI  (41)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (97)
1 site
A C T A G T T G A T C A
SnaBI  (438)
1 site
T A C G T A A T G C A T
BsmBI  (687)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
SalI  (753)
1 site
G T C G A C C A G C T G
AccI  (754)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PpuMI  (788)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (788)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
MfeI  (1130)
1 site
C A A T T G G T T A A C
PspOMI  (1305)
1 site
G G G C C C C C C G G G
ApaI  (1309)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (1411)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XhoI  (1417)
2 sites
C T C G A G G A G C T C
SwaI  (1572)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PasI  (2074)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (2078)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XcmI  (2184)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PstI  (2224)
1 site
C T G C A G G A C G T C
AgeI  (2417)
1 site
A C C G G T T G G C C A
Acc65I  (2444)
1 site
G G T A C C C C A T G G
KpnI  (2448)
1 site
G G T A C C C C A T G G
BclI  (2520)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BmgBI  (2578)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
Bpu10I  (2688)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (2697)
1 site
T T C G A A A A G C T T
XhoI  (3097)
2 sites
C T C G A G G A G C T C
BamHI  (3103)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Bsu36I  (3125)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
MscI  (3166)
1 site
T G G C C A A C C G G T
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 1:  signal peptide  
   1436 .. 1483  =  48 bp
   16 amino acids  =  1.8 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 2:  
   1484 .. 2971  =  1488 bp
   495 amino acids  =  55.7 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4837 .. 5628  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5629 .. 5697  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5698 .. 5802  =  105 bp
AmpR promoter
5698 .. 5802  =  105 bp
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  622 .. 909  =  288 bp
ORF:  95 amino acids  =  11.4 kDa
ORF:  1322 .. 2971  =  1650 bp
ORF:  549 amino acids  =  61.7 kDa
ORF:  4967 .. 5233  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1881 .. 2291  =  411 bp
ORF:  136 amino acids  =  15.2 kDa
ORF:  2392 .. 2640  =  249 bp
ORF:  82 amino acids  =  8.8 kDa
ORF:  4837 .. 5697  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1604 .. 1882  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
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Download pCMV-VSV-G.dna file

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Individual Sequences & Maps

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