Resources
Plasmid Files

pLVX-Het-2

Lentiviral vector encoding an FKBP heterodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLVX-Het-2 Sequence and MappLVX-Het-2.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 XcmI (8759) AanI (8572) SgrDI (8534) SspI (8417) FspI (7835) AhdI (7613) BmtI (6339) NheI (6335) StuI (6324) AanI (6288) Bsu36I (5545) PfoI (5097) MluI (4811) RsrII (4193) KasI (637) NarI (638) SfoI (639) PluTI (641) BssHII (711) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) PpuMI - SanDI (1934) cPPT/CTS BspDI - ClaI (2180) SnaBI (2480) PaeR7I - XhoI (2810) 5' MCS SpeI (2816) ATG XbaI (2825) TspMI - XmaI (2946) SmaI (2948) NotI (3154) 3' MCS BamHI (3161) SnaBI (3169) PspOMI (3292) ApaI (3296) AvrII (3330) AarI (3518) BmgBI (3722) PshAI (3812) AsiSI (4149) pLVX-Het-2 8902 bp
XcmI  (8759)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
AanI  (8572)
2 sites
T T A T A A A A T A T T
SgrDI  (8534)
1 site
C G T C G A C G G C A G C T G C
SspI  (8417)
1 site
A A T A T T T T A T A A
FspI  (7835)
1 site
T G C G C A A C G C G T
AhdI  (7613)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmtI  (6339)
1 site
G C T A G C C G A T C G
NheI  (6335)
1 site
G C T A G C C G A T C G
StuI  (6324)
1 site
A G G C C T T C C G G A
AanI  (6288)
2 sites
T T A T A A A A T A T T
Bsu36I  (5545)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PfoI  (5097)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
MluI  (4811)
1 site
A C G C G T T G C G C A
RsrII  (4193)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
KasI  (637)
1 site
G G C G C C C C G C G G
NarI  (638)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (639)
1 site
G G C G C C C C G C G G
PluTI  (641)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
BssHII  (711)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
SnaBI  (2480)
2 sites
T A C G T A A T G C A T
PaeR7I  (2810)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2810)
1 site
C T C G A G G A G C T C
SpeI  (2816)
1 site
A C T A G T T G A T C A
XbaI  (2825)
1 site
T C T A G A A G A T C T
TspMI  (2946)
1 site
C C C G G G G G G C C C
XmaI  (2946)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (2948)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
NotI  (3154)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3161)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SnaBI  (3169)
2 sites
T A C G T A A T G C A T
PspOMI  (3292)
1 site
G G G C C C C C C G G G
ApaI  (3296)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3330)
1 site
C C T A G G G G A T C C
AarI  (3518)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BmgBI  (3722)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
PshAI  (3812)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AsiSI  (4149)
1 site
G C G A T C G C C G C T A G C G
HygR
3791 .. 4810  =  1020 bp
339 amino acids  =  37.8 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
HygR
3791 .. 4810  =  1020 bp
339 amino acids  =  37.8 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
AmpR
7540 .. 8400  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7540 .. 8331  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7540 .. 8400  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   8332 .. 8400  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7540 .. 8400  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
3' LTR
5620 .. 6253  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5620 .. 6253  =  634 bp
3' long terminal repeat (LTR) from HIV-1
WPRE
4824 .. 5412  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
4824 .. 5412  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
6784 .. 7369  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6784 .. 7369  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
IRES
3179 .. 3752  =  574 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
IRES
3179 .. 3752  =  574 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
FKBP (DmrA)
2831 .. 3151  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
FKBP (DmrA)
2831 .. 3151  =  321 bp
107 amino acids  =  11.8 kDa
Product: human FK506-binding protein FKBP12
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
8553 .. 8687  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8553 .. 8687  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
AmpR promoter
8401 .. 8505  =  105 bp
AmpR promoter
8401 .. 8505  =  105 bp
3' MCS
3153 .. 3166  =  14 bp
multiple cloning site downstream of DmrA
3' MCS
3153 .. 3166  =  14 bp
multiple cloning site downstream of DmrA
5' MCS
2810 .. 2821  =  12 bp
multiple cloning site upstream of DmrA
5' MCS
2810 .. 2821  =  12 bp
multiple cloning site upstream of DmrA
ATG
2822 .. 2824  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2822 .. 2824  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter