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pLVX-Het-Nuc1

Lentiviral vector encoding an FKBP heterodimerizer domain, for creating nuclear fusion proteins that can be dimerized with a drug.

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pLVX-Het-Nuc1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (8780) SgrDI (8555) SspI (8438) FspI (7856) AhdI (7634) BmtI (6360) NheI (6356) StuI (6345) Bsu36I (5566) PfoI (5118) MluI (4832) RsrII (4214) KasI (637) NarI (638) SfoI (639) PluTI (641) BssHII (711) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) PaeR7I - XhoI (2810) SpeI (2816) XbaI (2846) TspMI - XmaI (2967) SmaI (2969) NotI (3175) BamHI (3182) PspOMI (3313) ApaI (3317) AvrII (3351) BmgBI (3743) PshAI (3833) AsiSI (4170) pLVX-Het-Nuc1 8923 bp
XcmI  (8780)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8555)
1 site
C G T C G A C G G C A G C T G C
SspI  (8438)
1 site
A A T A T T T T A T A A
FspI  (7856)
1 site
T G C G C A A C G C G T
AhdI  (7634)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmtI  (6360)
1 site
G C T A G C C G A T C G
NheI  (6356)
1 site
G C T A G C C G A T C G
StuI  (6345)
1 site
A G G C C T T C C G G A
Bsu36I  (5566)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PfoI  (5118)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
MluI  (4832)
1 site
A C G C G T T G C G C A
RsrII  (4214)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
KasI  (637)
1 site
G G C G C C C C G C G G
NarI  (638)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (639)
1 site
G G C G C C C C G C G G
PluTI  (641)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BssHII  (711)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
PaeR7I  (2810)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2810)
1 site
C T C G A G G A G C T C
SpeI  (2816)
1 site
A C T A G T T G A T C A
XbaI  (2846)
1 site
T C T A G A A G A T C T
TspMI  (2967)
1 site
C C C G G G G G G C C C
XmaI  (2967)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (2969)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
NotI  (3175)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3182)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PspOMI  (3313)
1 site
G G G C C C C C C G G G
ApaI  (3317)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3351)
1 site
C C T A G G G G A T C C
BmgBI  (3743)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PshAI  (3833)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AsiSI  (4170)
1 site