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pLVX-Hom-Mem1

Lentiviral vector encoding FKBP homodimerizer domains, for creating membrane-associated fusion proteins that can be dimerized with a drug. 

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pLVX-Hom-Mem1 Sequence and MappLVX-Hom-Mem1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 XcmI (8698) SgrDI (8473) SspI (8356) PvuI (7922) FspI (7774) AhdI (7552) DrdI (6770) BmtI (6278) NheI (6274) PstI (5368) MluI (4750) SexAI * (4717) MscI * (4525) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) SanDI (1934) BspDI - ClaI (2180) CMV enhancer NdeI (2374) SnaBI (2480) EcoRI (2803) 5' MCS SpeI (2815) AfeI (2860) XbaI (2863) BlpI (3083) NotI (3519) 3' MCS BamHI (3526) SnaBI (3534) PspOMI (3657) ApaI (3661) AvrII (3695) BmgBI (4087) PflFI - Tth111I (4192) BsiWI (4206) RsrII (4266) BstEII (4284) pLVX-Hom-Mem1 8841 bp
XcmI  (8698)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8473)
1 site
C G T C G A C G G C A G C T G C
SspI  (8356)
1 site
A A T A T T T T A T A A
PvuI  (7922)
1 site
C G A T C G G C T A G C
FspI  (7774)
1 site
T G C G C A A C G C G T
AhdI  (7552)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6770)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6278)
1 site
G C T A G C C G A T C G
NheI  (6274)
1 site
G C T A G C C G A T C G
PstI  (5368)
1 site
C T G C A G G A C G T C
MluI  (4750)
1 site
A C G C G T T G C G C A
SexAI  (4717)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (4525)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
SanDI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (2480)
2 sites
T A C G T A A T G C A T
EcoRI  (2803)
1 site
G A A T T C C T T A A G
SpeI  (2815)
1 site
A C T A G T T G A T C A
AfeI  (2860)
1 site
A G C G C T T C G C G A
XbaI  (2863)
1 site
T C T A G A A G A T C T
BlpI  (3083)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (3519)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3526)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SnaBI  (3534)
2 sites
T A C G T A A T G C A T
PspOMI  (3657)
1 site
G G G C C C C C C G G G
ApaI  (3661)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3695)
1 site
C C T A G G G G A T C C
BmgBI  (4087)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
PflFI  (4192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (4206)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (4266)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (4284)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AmpR
7479 .. 8339  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7479 .. 8270  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7479 .. 8339  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   8271 .. 8339  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7479 .. 8339  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
3' LTR
5559 .. 6192  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5559 .. 6192  =  634 bp
3' long terminal repeat (LTR) from HIV-1
PuroR
4150 .. 4749  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4150 .. 4749  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
4763 .. 5351  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
4763 .. 5351  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
6723 .. 7308  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6723 .. 7308  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
IRES
3544 .. 4117  =  574 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
IRES
3544 .. 4117  =  574 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
DmrB
2869 .. 3189  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein
FKBP12
binds synthetic ligands that do not bind wild-type
FKBP
DmrB
2869 .. 3189  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein
FKBP12
binds synthetic ligands that do not bind wild-type
FKBP
DmrB
3196 .. 3516  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein
FKBP12
binds synthetic ligands that do not bind wild-type
FKBP
DmrB
3196 .. 3516  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein
FKBP12
binds synthetic ligands that do not bind wild-type
FKBP
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
8492 .. 8626  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8492 .. 8626  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)
AmpR promoter
8340 .. 8444  =  105 bp
AmpR promoter
8340 .. 8444  =  105 bp
N-myristoylation signal
2821 .. 2862  =  42 bp
14 amino acids  =  1.5 kDa
Product: N-myristoylation signal from Src kinase
N-myristoylation signal
2821 .. 2862  =  42 bp
14 amino acids  =  1.5 kDa
Product: N-myristoylation signal from Src kinase
5' MCS
2803 .. 2820  =  18 bp
multiple cloning site upstream of DmrB
5' MCS
2803 .. 2820  =  18 bp
multiple cloning site upstream of DmrB
3' MCS
3518 .. 3531  =  14 bp
multiple cloning site downstream of DmrB
3' MCS
3518 .. 3531  =  14 bp
multiple cloning site downstream of DmrB
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