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Plasmid Files

pLVX-Hom-Nuc1

Lentiviral vector encoding an FKBP homodimerizer domain, for creating nuclear fusion proteins that can be dimerized with a drug.

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pLVX-Hom-Nuc1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (8356) SgrDI (8131) SspI (8014) PvuI (7580) FspI (7432) AhdI (7210) DrdI (6428) BmtI (5936) NheI (5932) Bsu36I (5142) PstI (5026) MluI (4408) SexAI * (4375) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2803) PaeR7I - XhoI (2809) SpeI (2815) XbaI (2848) NotI (3177) BamHI (3184) PspOMI (3315) ApaI (3319) AvrII (3353) BmgBI (3745) PflFI - Tth111I (3850) BsiWI (3864) RsrII (3924) BstEII (3942) MscI * (4183) pLVX-Hom-Nuc1 8499 bp
XcmI  (8356)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8131)
1 site
C G T C G A C G G C A G C T G C
SspI  (8014)
1 site
A A T A T T T T A T A A
PvuI  (7580)
1 site
C G A T C G G C T A G C
FspI  (7432)
1 site
T G C G C A A C G C G T
AhdI  (7210)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6428)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (5936)
1 site
G C T A G C C G A T C G
NheI  (5932)
1 site
G C T A G C C G A T C G
Bsu36I  (5142)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (5026)
1 site
C T G C A G G A C G T C
MluI  (4408)
1 site
A C G C G T T G C G C A
SexAI  (4375)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
EcoRI  (2803)
1 site
G A A T T C C T T A A G
PaeR7I  (2809)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2809)
1 site
C T C G A G G A G C T C
SpeI  (2815)
1 site
A C T A G T T G A T C A
XbaI  (2848)
1 site
T C T A G A A G A T C T
NotI  (3177)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3184)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PspOMI  (3315)
1 site
G G G C C C C C C G G G
ApaI  (3319)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3353)
1 site
C C T A G G G G A T C C
BmgBI  (3745)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PflFI  (3850)
1 site
G A C N N