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pLVX-TRE3G-mCherry

Lentiviral vector for doxycycline-inducible expression of a gene together with the mCherry fluorescent protein.

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pLVX-TRE3G-mCherry.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SgrDI (8747) SspI (8630) PvuI (8196) FspI (8048) BmtI (6552) NheI (6548) Bsu36I (5758) SexAI * (4987) RsrII (4536) NruI * (833) EcoNI (1170) MfeI (1189) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) SbfI (2919) SgrAI (3245) BsrGI (3264) SfiI (3282) PspOMI (3404) ApaI (3408) AvrII (3442) AarI (3630) TspMI - XmaI (3872) SmaI (3874) MluI (3878) NdeI (3885) EcoRI (3890) SphI (3939) AgeI (4010) SpeI (4145) BlpI (4173) PflFI - Tth111I (4462) PasI (4469) BsiWI (4476) pLVX-TRE3G-mCherry 9115 bp
SgrDI  (8747)
1 site
C G T C G A C G G C A G C T G C
SspI  (8630)
1 site
A A T A T T T T A T A A
PvuI  (8196)
1 site
C G A T C G G C T A G C
FspI  (8048)
1 site
T G C G C A A C G C G T
BmtI  (6552)
1 site
G C T A G C C G A T C G
NheI  (6548)
1 site
G C T A G C C G A T C G
Bsu36I  (5758)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SexAI  (4987)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
RsrII  (4536)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
PaeR7I  (2203)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2203)
1 site
V C T C G A G