Resources
Plasmid Files

pLVX-Tight-Puro

Lentiviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLVX-Tight-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
Download Free Trial Get SnapGene Viewer


SgrDI (7423) SspI (7306) PvuI (6872) FspI (6724) AhdI (6502) PciI (5609) BmtI (5226) NheI (5222) KpnI (4440) Acc65I (4436) Bsu36I (4432) NcoI (4120) PflMI (3953) FseI (1150) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) BamHI (2528) NotI (2535) XbaI * (2548) MluI (2560) EcoRI (2566) SphI (2615) AgeI (2686) SpeI (2821) BlpI (2849) BfuAI - BspMI (3085) PflFI - Tth111I (3138) PasI (3145) BsiWI (3152) RsrII (3212) BstEII (3230) MscI * (3471) SexAI * (3663) pLVX-Tight-Puro 7790 bp
SgrDI  (7423)
1 site
C G T C G A C G G C A G C T G C
SspI  (7306)
1 site
A A T A T T T T A T A A
PvuI  (6872)
1 site
C G A T C G G C T A G C
FspI  (6724)
1 site
T G C G C A A C G C G T
AhdI  (6502)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5609)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BmtI  (5226)
1 site
G C T A G C C G A T C