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Plasmid Files

pLVX-rHom-Nuc1

Lentiviral vector encoding three FKBP homodimerizer domains, for creating nuclear oligomers that can be dissociated with a drug. 

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pLVX-rHom-Nuc1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (9017) SgrDI (8792) SspI (8675) PvuI (8241) FspI (8093) AhdI (7871) DrdI (7089) BmtI (6597) NheI (6593) Bsu36I (5803) PstI (5687) MluI (5069) SexAI * (5036) MscI * (4844) BstEII (4603) RsrII (4585) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2804) PaeR7I - XhoI (2810) XbaI (2846) NotI (3838) BamHI (3845) PspOMI (3976) ApaI (3980) AvrII (4014) BmgBI (4406) PflFI - Tth111I (4511) BsiWI (4525) pLVX-rHom-Nuc1 9160 bp
XcmI  (9017)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8792)
1 site
C G T C G A C G G C A G C T G C
SspI  (8675)
1 site
A A T A T T T T A T A A
PvuI  (8241)
1 site
C G A T C G G C T A G C
FspI  (8093)
1 site
T G C G C A A C G C G T
AhdI  (7871)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (7089)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6597)
1 site
G C T A G C C G A T C G
NheI  (6593)
1 site
G C T A G C C G A T C G
Bsu36I  (5803)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (5687)
1 site
C T G C A G G A C G T C
MluI  (5069)
1 site
A C G C G T T G C G C A
SexAI  (5036)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MscI  (4844)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
BstEII  (4603)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
RsrII  (4585)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G