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Plasmid Files

pLVX-rHom-Sec1

Lentiviral vector encoding four FKBP homodimerizer domains, for creating ER-localized oligomers that can be dissociated with a drug. 

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pLVX-rHom-Sec1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (9407) SgrDI (9182) SspI (9065) PvuI (8631) FspI (8483) AhdI (8261) DrdI (7479) BmtI (6987) NheI (6983) Bsu36I (6193) PstI (6077) MluI (5459) SexAI * (5426) MscI * (5234) BstEII (4993) RsrII (4975) BsiWI (4915) PflFI - Tth111I (4901) BmgBI (4796) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2804) PaeR7I - XhoI (2810) XbaI (2900) NotI (4228) BamHI (4235) PspOMI (4366) ApaI (4370) AvrII (4404) pLVX-rHom-Sec1 9550 bp
XcmI  (9407)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (9182)
1 site
C G T C G A C G G C A G C T G C
SspI  (9065)
1 site
A A T A T T T T A T A A
PvuI  (8631)
1 site
C G A T C G G C T A G C
FspI  (8483)
1 site
T G C G C A A C G C G T
AhdI  (8261)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (7479)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (6987)
1 site
G C T A G C C G A T C G
NheI  (6983)
1 site
G C T A G C C G A T C G
Bsu36I  (6193)
1 site
C C T N A G G G G A N