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pLenti-C-Myc-DDK-IRES-tRFP

Third-generation lentiviral PrecisionShuttle™ vector with a TurboRFP marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.

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pLenti-C-Myc-DDK-IRES-tRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsmBI (7120) DrdI (6285) SacII (5382) PshAI (4692) SfiI (4528) BmgBI (4033) AarI (3829) SgrDI (1) KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) BspQI - SapI (1482) FseI (1530) EcoNI (1550) BbvCI (1804) AleI (1957) PstI (2417) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI - SgfI (3324) MreI - SgrAI (3326) AscI (3330) NheI (3355) BmtI (3359) RsrII (3361) MluI (3367) BsiWI (3370) PaeR7I - PspXI - XhoI (3382) PmeI (3460) PmlI (3806) pLenti-C-Myc-DDK-IRES-tRFP 7695 bp
BsmBI  (7120)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
DrdI  (6285)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
SacII  (5382)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PshAI  (4692)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SfiI  (4528)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BmgBI  (4033)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
AarI  (3829)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
KasI  (1017)
1 site
G G C G C C C C G C G G
NarI  (1018)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1019)
1 site
G G C G C C C C G C G G
PluTI  (1021)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BspQI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FseI  (1530)
1 site
G G C