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pLenti-C-tRFP

Third-generation lentiviral PrecisionShuttle™ vector for expression of an ORF tagged at the C-terminus with TurboRFP. Also known as pLenti-C-RFP.

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pLenti-C-tRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AclI (6604) BsmBI (6465) DrdI (5630) PfoI (4497) PmeI (4087) PshAI (4015) BfuAI - BspMI (3902) SfiI (3851) BclI * (3398) KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) XmnI (1224) BspQI - SapI (1482) EcoNI (1550) BbvCI (1804) AleI (1957) SanDI (2314) PstI (2417) PspOMI (2605) ApaI (2609) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI - SgfI (3324) MreI - SgrAI (3326) AscI (3330) RsrII (3361) MluI (3367) BsiWI (3370) NotI (3376) PaeR7I - XhoI (3382) pLenti-C-tRFP 7006 bp
AclI  (6604)
1 site
A A C G T T T T G C A A
BsmBI  (6465)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
DrdI  (5630)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PfoI  (4497)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PmeI  (4087)
1 site
G T T T A A A C C A A A T T T G
PshAI  (4015)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BfuAI  (3902)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3902)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SfiI  (3851)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BclI  (3398)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
KasI  (1017)
1 site
G G C G C C C C G C G G
NarI  (1018)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1019)
1 site
G G C G C C C C G C G G
PluTI  (1021)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XmnI  (1224)
1 site
G A A N N N N T T C C T T N N N N A A G
BspQI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
AleI  (1957)
1 site
C A C N N N N G T G G T G N N N N C A C
SanDI  (2314)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
PstI  (2417)
1 site
C T G C A G G A C G T C
PspOMI  (2605)
1 site
G G G C C C C C C G G G
ApaI  (2609)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
XbaI  (2613)
1 site
T C T A G A A G A T C T
EcoRI  (3279)
1 site
G A A T T C C T T A A G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AsiSI  (3324)
1 site
G C G A T C G C C G C T A G C G
SgfI  (3324)
1 site
G C G A T C G C C G C T A G C G
MreI  (3326)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (3326)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (3330)
1 site
G G C G C G C C C C G C G C G G
RsrII  (3361)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (3367)
1 site
A C G C G T T G C G C A
BsiWI  (3370)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NotI  (3376)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (3382)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (3382)
1 site