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pLenti6/TR

Lentiviral vector for high-level constitutive expression of the Tet repressor.

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pLenti6 TR.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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KasI (413) PciI (7944) AhdI (7056) DraIII (5916) NaeI (5810) NgoMIV (5808) SfiI (5428) BlpI (4785) BsaBI * (4688) MscI (4393) PmlI (4321) NarI (414) SfoI (415) PluTI (417) AccI (738) NotI (920) BbvCI (1196) BsmBI (2344) SpeI (2406) BstXI (2419) PstI (2432) BspEI * (2526) AfeI (3612) SacII (3864) BstBI (3868) AgeI (3909) SexAI * (4028) pLenti6/TR 8354 bp
KasI  (413)
1 site
G G C G C C C C G C G G
PciI  (7944)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (7056)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (5916)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (5810)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5808)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SfiI  (5428)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BlpI  (4785)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsaBI  (4688)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MscI  (4393)
1 site
T G G C C A A C C G G T
PmlI  (4321)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
NarI  (414)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (415)
1 site
G G C G C C C C G C G G
PluTI  (417)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
AccI  (738)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NotI  (920)
1 site
G C G G C C G C C G C C G G C G
BbvCI  (1196)
1 site
C C T C A G C G G A G T C G
BsmBI  (2344)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (2406)
1 site
A C T