pMD2.G

Lentiviral envelope plasmid from the Trono lab.

Sequence Author: Addgene

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No matches
EcoNI (5776) PasI (5772) PflMI (5583) DraIII (5580) PmlI (5111) KflI - PpuMI (4595) HindIII (4541) BsmBI - Esp3I (4471) SnaBI (4222) BtgZI (4216) HincII (3866) BsrGI (3825) ApaI (3724) PspOMI (3720) Bsu36I (3714) XmnI (3383) ScaI (3264) PvuI (3154) FspI (3006) BstAPI (12) XcmI (60) PstI (100) AgeI (293) BclI * (396) Bpu10I (564) BstBI (573) BspQI - SapI (1255) MscI * (1608) AccI (1646) EagI - NotI (1763) HaeII (2139) BseYI (2195) PspFI (2199) AhdI (2784) pMD2.G 5822 bp
EcoNI  (5776)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PasI  (5772)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (5583)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
DraIII  (5580)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PmlI  (5111)
1 site
C A C G T G G T G C A C
KflI  (4595)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (4595)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
HindIII  (4541)
1 site
A A G C T T T T C G A A
BsmBI  (4471)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (4471)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
SnaBI  (4222)
1 site
T A C G T A A T G C A T
BtgZI  (4216)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
HincII  (3866)
1 site
G T Y R A C C A R Y T G
BsrGI  (3825)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
ApaI  (3724)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3720)
1 site
G G G C C C C C C G G G
Bsu36I  (3714)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
XmnI  (3383)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3264)
1 site
A G T A C T T C A T G A
PvuI  (3154)
1 site
C G A T C G G C T A G C
FspI  (3006)
1 site
T G C G C A A C G C G T
BstAPI  (12)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
XcmI  (60)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PstI  (100)
1 site
C T G C A G G A C G T C
AgeI  (293)
1 site
A C C G G T T G G C C A
BclI  (396)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Bpu10I  (564)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (573)
1 site
T T C G A A A A G C T T
BspQI  (1255)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1255)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
MscI  (1608)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
AccI  (1646)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
EagI  (1763)
1 site
C G G C C G G C C G G C
NotI  (1763)
1 site
G C G G C C G C C G C C G G C G
HaeII  (2139)
1 site
R G C G C Y Y C G C G R
BseYI  (2195)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (2199)
1 site
C C C A G C G G G T C G
AhdI  (2784)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
VSV-G
5134 .. 847  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 1:  signal peptide  
   5134 .. 5181  =  48 bp
   16 amino acids  =  1.8 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
5134 .. 847  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 2:  
   5182 .. 847  =  1488 bp
   495 amino acids  =  55.7 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
5134 .. 847  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
AmpR
2711 .. 3571  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2711 .. 3502  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2711 .. 3571  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3503 .. 3571  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2711 .. 3571  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1952 .. 2540  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1952 .. 2540  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
4584 .. 5059  =  476 bp
internally truncated intron from human β-globin
β-globin intron
4584 .. 5059  =  476 bp
internally truncated intron from human β-globin
β-globin poly(A)
1048 .. 1442  =  395 bp
human β-globin polyadenylation signal
β-globin poly(A)
1048 .. 1442  =  395 bp
human β-globin polyadenylation signal
CMV enhancer
3867 .. 4246  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3867 .. 4246  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
4247 .. 4450  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4247 .. 4450  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
3572 .. 3676  =  105 bp
AmpR promoter
3572 .. 3676  =  105 bp
ORF:  5134 .. 847  =  1536 bp
ORF:  511 amino acids  =  57.5 kDa
ORF:  5579 .. 167  =  411 bp
ORF:  136 amino acids  =  15.2 kDa
ORF:  2841 .. 3107  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2711 .. 3571  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  268 .. 516  =  249 bp
ORF:  82 amino acids  =  8.8 kDa
ORF:  5302 .. 5580  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
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