Resources
Plasmid Files

pMSCVneo

Retroviral vector with a neomycin (G418) resistance marker optimized for gene expression in hematopoietic, embryonic stem, or embryonic carcinoma cells.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pMSCVneo Sequence and MappMSCVneo.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 SgrAI (6258) NdeI (5886) PfoI (5748) ScaI (5195) AflIII - PciI (3822) BstEII (1088) SexAI * (1216) BsrGI (1283) 5' pMSCV primer (1333 .. 1355) EcoRI (1405) HpaI (1413) PaeR7I - XhoI (1417) BglII (1423) AgeI (1548) 3' pMSCV primer (1660 .. 1682) BlpI (1711) StuI (1732) BspEI (1857) BsmI (1869) BsaAI (2392) NcoI (2520) RsrII (2604) BamHI (2757) SalI (2763) AccI (2764) HindIII (2777) BspDI - ClaI (2784) pMSCVneo 6436 bp
SgrAI  (6258)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
NdeI  (5886)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
PfoI  (5748)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
ScaI  (5195)
1 site
A G T A C T T C A T G A
AflIII  (3822)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3822)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstEII  (1088)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
SexAI  (1216)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BsrGI  (1283)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (1405)
1 site
G A A T T C C T T A A G
HpaI  (1413)
1 site
G T T A A C C A A T T G
PaeR7I  (1417)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1417)
1 site
C T C G A G G A G C T C
BglII  (1423)
1 site
A G A T C T T C T A G A
AgeI  (1548)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BlpI  (1711)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
StuI  (1732)
1 site
A G G C C T T C C G G A
BspEI  (1857)
1 site
T C C G G A A G G C C T
BsmI  (1869)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsaAI  (2392)
1 site
Y A C G T R R T G C A Y
NcoI  (2520)
1 site
C C A T G G G G T A C C
RsrII  (2604)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BamHI  (2757)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SalI  (2763)
1 site
G T C G A C C A G C T G
AccI  (2764)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HindIII  (2777)
1 site
A A G C T T T T C G A A
BspDI  (2784)
1 site
A T C G A T T A G C T A
ClaI  (2784)
1 site
A T C G A T T A G C T A
5' pMSCV primer
23-mer  /  61% GC
1 binding site
1333 .. 1355  =  23 annealed bases
Tm  =  62°C
3' pMSCV primer
23-mer  /  48% GC
1 binding site
1660 .. 1682  =  23 annealed bases
Tm  =  58°C
AmpR
4642 .. 5502  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4642 .. 5433  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4642 .. 5502  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5434 .. 5502  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4642 .. 5502  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
NeoR/KanR
1951 .. 2754  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
1951 .. 2754  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
ori
3883 .. 4471  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3883 .. 4471  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
5' LTR
6435 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem
cell virus
5' LTR
6435 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem
cell virus
3' LTR
2829 .. 3343  =  515 bp
3' long terminal repeat from murine embryonic stem
cell virus
3' LTR
2829 .. 3343  =  515 bp
3' long terminal repeat from murine embryonic stem
cell virus
PGK promoter
1438 .. 1937  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
1438 .. 1937  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell
virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell
virus
AmpR promoter
5503 .. 5607  =  105 bp
AmpR promoter
5503 .. 5607  =  105 bp
MCS
1405 .. 1428  =  24 bp
multiple cloning site
MCS
1405 .. 1428  =  24 bp
multiple cloning site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter