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pRetroQ-AcGFP1-N1

Self-inactivating retroviral vector for expressing a protein fused to the N-terminus of AcGFP1.

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pRetroQ-AcGFP1-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SspI (7261) XmnI (7056) ScaI (6937) FspI (6679) AflIII - PciI (5564) BspQI - SapI (5448) AvrII (5317) SfiI (5270) EcoRV (4193) RsrII (3637) AscI (542) PshAI (676) NheI (2172) BmtI (2176) BglII (2190) PaeR7I - XhoI (2194) BstBI (2208) EcoRI (2210) SalI (2220) AccI (2221) PspOMI (2234) ApaI (2238) BamHI (2241) AleI (2434) PflMI (2952) NotI (2975) XbaI (2985) BlpI (3274) BsmI (3432) BfuAI - BspMI (3510) BsiWI (3577) pRetroQ-AcGFP1-N1 7606 bp
SspI  (7261)
1 site
A A T A T T T T A T A A
XmnI  (7056)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6937)
1 site
A G T A C T T C A T G A
FspI  (6679)
1 site
T G C G C A A C G C G T
AflIII  (5564)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5564)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5448)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5448)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5317)
1 site
C C T A G G G G A T C C
SfiI  (5270)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (4193)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
RsrII  (3637)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (542)
1 site
G G C G C G C C C C G C G C G G
PshAI  (676)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NheI  (2172)
1 site
G C T A G C C G A T C G
BmtI  (2176)
1 site
G C T A G C C G A T C G
BglII  (2190)
1 site
A G A T C T T C T A G A
PaeR7I  (2194)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2194)
1 site
C T C G A G G A G C T C
BstBI  (2208)
1 site
T T C G A A A A G C T T
EcoRI  (2210)
1 site
G A A T T C C T T A A G
SalI  (2220)
1 site
G T C G A C C A G C T G
AccI  (2221)
1 site
G T M K A C C A K M T G