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Plasmid Files

pRetroQ-mCherry-N1

Self-inactivating retroviral vector for expressing a protein fused to the N-terminus of mCherry.

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pRetroQ-mCherry-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SspI (7252) XmnI (7047) ScaI (6928) FspI (6670) AflIII - PciI (5555) BspQI - SapI (5439) AvrII (5308) SfiI (5261) EcoRV (4184) DraIII (4083) RsrII (3628) AscI (542) PshAI (676) NheI (2172) BmtI (2176) BglII (2190) PaeR7I - XhoI (2194) BstBI (2208) EcoRI (2210) SalI (2220) AccI (2221) PspOMI (2234) ApaI (2238) BamHI (2241) SbfI (2609) PflMI (2693) BbvCI (2792) AleI (2896) SgrAI (2935) XcmI (2940) NotI (2966) XbaI (2976) BlpI (3265) BsmI (3423) BfuAI - BspMI (3501) BsiWI (3568) pRetroQ-mCherry-N1 7597 bp
SspI  (7252)
1 site
A A T A T T T T A T A A
XmnI  (7047)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6928)
1 site
A G T A C T T C A T G A
FspI  (6670)
1 site
T G C G C A A C G C G T
AflIII  (5555)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5555)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5439)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5439)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5308)
1 site
C C T A G G G G A T C C
SfiI  (5261)
1 site