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pRetroX-TetOne™

All-in-one retroviral vector for strong inducible expression of genes using the Tet-On® system.

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pRetroX-TetOne.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BspQI - SapI (8267) PciI (8150) AlwNI (7741) ScaI (6781) SspI (6457) SacII (5120) AleI (5119) MluI (5092) EcoRV (4734) PflMI (4557) BsiWI (4433) AvrII (77) SnaBI (519) AfeI (717) Eco53kI (733) SacI (735) PshAI (1170) SrfI (1394) AflII (1416) Bsu36I (1428) BstEII (1497) Bpu10I (1700) PpuMI (2077) BamHI (2607) NgoMIV (2613) NaeI (2615) SgrAI (2619) BstZ17I (2627) EcoRI (2633) HindIII (2752) PaeR7I - PspXI - XhoI (3004) AscI (3368) BspEI (3473) NruI (3832) BfuAI - BspMI (4109) pRetroX-TetOne™ 8508 bp
BspQI  (8267)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (8267)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (8150)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (7741)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ScaI  (6781)
1 site
A G T A C T T C A T G A
SspI  (6457)
1 site
A A T A T T T T A T A A
SacII  (5120)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (5119)
1 site
C A C N N N N G T G G T G N N N N C A C
MluI  (5092)
1 site
A C G C G T T G C G C A
EcoRV  (4734)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PflMI  (4557)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsiWI  (4433)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
AvrII  (77)
1 site
C C T A G G G G A T C C
SnaBI