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Plasmid Files

YRp7

Yeast replicating vector with a TRP1 marker.

 
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 AatII (5747) ZraI (5745) SspI (5629) ScaI (5305) PvuI (5195) BsaI (4886) AhdI (4825) AlwNI (4348) BspQI - SapI (3816) NdeI (3755) PflFI - Tth111I (3679) PfoI (3576) BsmBI (3575) PvuII (3525) BspEI * (3121) Bpu10I (3038) MscI * (2903) DraIII (45) BglII (603) StuI (630) SbfI (636) AarI (920) BstXI (1063) Bsu36I (1112) MfeI (1213) XbaI (1269) PmlI (1397) BspDI - ClaI (1481) BamHI (1832) SgrAI (1867) SphI (2023) EcoNI (2083) SalI (2108) PshAI (2173) EagI (2396) NruI (2431) BsmI (2816) StyI (2826) AvaI - BsoBI (2882) BmeT110I (2883) YRp7 5817 bp
AatII  (5747)
1 site
G A C G T C C T G C A G
ZraI  (5745)
1 site
G A C G T C C T G C A G
SspI  (5629)
1 site
A A T A T T T T A T A A
ScaI  (5305)
1 site
A G T A C T T C A T G A
PvuI  (5195)
1 site
C G A T C G G C T A G C
BsaI  (4886)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4825)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4348)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspQI  (3816)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3816)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
NdeI  (3755)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
PflFI  (3679)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3679)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (3576)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BsmBI  (3575)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PvuII  (3525)
1 site
C A G C T G G T C G A C
BspEI  (3121)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
Bpu10I  (3038)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
MscI  (2903)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
DraIII  (45)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BglII  (603)
1 site
A G A T C T T C T A G A
StuI  (630)
1 site
A G G C C T T C C G G A
SbfI  (636)
1 site
C C T G C A G G G G A C G T C C
AarI  (920)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BstXI  (1063)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
Bsu36I  (1112)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
MfeI  (1213)
1 site
C A A T T G G T T A A C
XbaI  (1269)
1 site
T C T A G A A G A T C T
PmlI  (1397)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BspDI  (1481)
1 site
A T C G A T T A G C T A
ClaI  (1481)
1 site
A T C G A T T A G C T A
BamHI  (1832)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (1867)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SphI  (2023)
1 site
G C A T G C C G T A C G
EcoNI  (2083)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SalI  (2108)
1 site
G T C G A C C A G C T G
PshAI  (2173)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
EagI  (2396)
1 site
C G G C C G G C C G G C
NruI  (2431)
1 site
T C G C G A A G C G C T
BsmI  (2816)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
StyI  (2826)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AvaI  (2882)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2882)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmeT110I  (2883)
1 site
C Y C G R G G R G C Y C
TcR
1543 .. 2733  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
1543 .. 2733  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
AmpR
4752 .. 5612  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   4752 .. 5543  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4752 .. 5612  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   5544 .. 5612  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4752 .. 5612  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ARS1
7 .. 844  =  838 bp
S. cerevisiae autonomously replicating sequence
ARS1/ARS416
ARS1
7 .. 844  =  838 bp
S. cerevisiae autonomously replicating sequence
ARS1/ARS416
ori
3993 .. 4581  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
ori
3993 .. 4581  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin
of replication
rop
3374 .. 3565  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
rop
3374 .. 3565  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
AmpR promoter
5613 .. 5717  =  105 bp
AmpR promoter
5613 .. 5717  =  105 bp
TRP1 promoter
1358 .. 1459  =  102 bp
TRP1 promoter
1358 .. 1459  =  102 bp
tet promoter
1467 .. 1495  =  29 bp
   Segment 1:  -35  
   1467 .. 1472  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
1467 .. 1495  =  29 bp
   Segment 2:  
   1473 .. 1489  =  17 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
1467 .. 1495  =  29 bp
   Segment 3:  -10  
   1490 .. 1495  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
1467 .. 1495  =  29 bp
3 segments
E. coli promoter for tetracycline efflux protein gene
TRP1
683 .. 1357  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase,
required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
683 .. 1357  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase,
required for tryptophan biosynthesis
yeast auxotrophic marker
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