Resources
Plasmid Files

hphMX6

hphMX6 selector module conferring hygromycin resistance, for gene disruption in yeast.

 
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 1500 1000 500 hphMX6 TEF promoter HygR TEF terminator End (1576) BsmI (1563) BtgZI (1020) RsrII (756) AsiSI (712) EcoRI (591) PshAI (375) MluI (203) BseRI (150) Bpu10I (48) BmgBI (39) Start (0) hphMX6 1576 bp
End  (1576)
0 sites
BsmI  (1563)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BtgZI  (1020)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
RsrII  (756)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AsiSI  (712)
1 site
G C G A T C G C C G C T A G C G
EcoRI  (591)
1 site
G A A T T C C T T A A G
PshAI  (375)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MluI  (203)
1 site
A C G C G T T G C G C A
BseRI  (150)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Bpu10I  (48)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BmgBI  (39)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
Start  (0)
0 sites
hphMX6
1 .. 1576  =  1576 bp
yeast selectable marker conferring hygromycin
resistance
hphMX6
1 .. 1576  =  1576 bp
yeast selectable marker conferring hygromycin
resistance
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
HygR
345 .. 1373  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
HygR
345 .. 1373  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
TEF terminator
1379 .. 1576  =  198 bp
Ashbya gossypii TEF terminator
TEF terminator
1379 .. 1576  =  198 bp
Ashbya gossypii TEF terminator
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