Resources
Plasmid Files

pDH25

Plasmid for transforming fungi to hygromycin resistance.

 
To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

 XbaI (6987) PasI (6748) BseRI (6690) BlpI (6566) SacII (6015) RsrII (5643) AsiSI (5599) BspDI - ClaI (5230) AflII (5167) SalI (4869) SmaI (4579) TspMI - XmaI (4577) BclI * (4546) EcoRV (4479) BmgBI (4339) BstBI (4017) SspI (3795) SgrAI (35) SphI (191) EcoNI (251) SalI (276) NruI (599) MscI * (1071) FspAI (1081) Bpu10I (1206) PvuII (1691) BsaAI (1852) BspQI - SapI (1982) PciI (2098) AlwNI (2514) AhdI (2991) pDH25 6993 bp
XbaI  (6987)
1 site
T C T A G A A G A T C T
PasI  (6748)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BseRI  (6690)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BlpI  (6566)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SacII  (6015)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
RsrII  (5643)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AsiSI  (5599)
1 site
G C G A T C G C C G C T A G C G
BspDI  (5230)
1 site
A T C G A T T A G C T A
ClaI  (5230)
1 site
A T C G A T T A G C T A
AflII  (5167)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
SalI  (4869)
2 sites
G T C G A C C A G C T G
SmaI  (4579)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (4577)
1 site
C C C G G G G G G C C C
XmaI  (4577)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BclI  (4546)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
EcoRV  (4479)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BmgBI  (4339)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BstBI  (4017)
1 site
T T C G A A A A G C T T
SspI  (3795)
1 site
A A T A T T T T A T A A
SgrAI  (35)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SphI  (191)
1 site
G C A T G C C G T A C G
EcoNI  (251)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SalI  (276)
2 sites
G T C G A C C A G C T G
NruI  (599)
1 site
T C G C G A A G C G C T
MscI  (1071)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
FspAI  (1081)
1 site
R T G C G C A Y Y A C G C G T R
Bpu10I  (1206)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (1691)
1 site
C A G C T G G T C G A C
BsaAI  (1852)
1 site
Y A C G T R R T G C A Y
BspQI  (1982)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1982)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
PciI  (2098)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (2514)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2991)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
HygR
5235 .. 6260  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
HygR
5235 .. 6260  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E.
coli

confers resistance to hygromycin
AmpR
2918 .. 3778  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   2918 .. 3709  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2918 .. 3778  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   3710 .. 3778  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2918 .. 3778  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2159 .. 2747  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2159 .. 2747  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
trpC terminator
6429 .. 6987  =  559 bp
terminator for Aspergillus nidulans trpC
trpC terminator
6429 .. 6987  =  559 bp
terminator for Aspergillus nidulans trpC
trpC promoter
4870 .. 5230  =  361 bp
promoter for Aspergillus nidulans trpC
trpC promoter
4870 .. 5230  =  361 bp
promoter for Aspergillus nidulans trpC
rop
1540 .. 1731  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at
low copy number
rop
1540 .. 1731  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at
low copy number
AmpR promoter
3779 .. 3883  =  105 bp
AmpR promoter
3779 .. 3883  =  105 bp
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter