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pESC-HIS

Yeast episomal vector with a HIS3 marker, for galactose-regulated expression and tagging of up to two genes.

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pESC-HIS.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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MfeI (6588) XcmI (6587) BmgBI (6376) XbaI (6275) SnaBI (5938) ScaI (4858) BpmI (4448) BsaI (4439) BmrI (4418) AhdI (4378) AlwNI (3901) PspFI (3793) BseYI (3789) BspQI - SapI (3369) PfoI (46) BsaBI * (561) NdeI (694) MscI (711) BsmI (716) BsiWI (916) BclI * (1052) AleI (1063) PstI (1187) BssHII (1312) DraIII (1647) NgoMIV (1748) NaeI (1750) PacI (2260) Eco53kI (2266) SacI (2268) BspDI - ClaI (2300) SpeI (2305) EagI - NotI (2312) EcoRI (2334) AgeI (2629) BstAPI (2881) BamHI (3012) PspOMI (3036) TspMI - XmaI (3039) ApaI (3040) SmaI - SrfI (3041) SalI (3046) PaeR7I - PspXI - XhoI (3082) BtgI (3099) SacII (3102) PpuMI (3159) BsrGI (3233) MluI (3240) pESC-HIS 6706 bp
MfeI  (6588)
1 site
C A A T T G G T T A A C
XcmI  (6587)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BmgBI  (6376)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
XbaI  (6275)
1 site
T C T A G A A G A T C T
SnaBI  (5938)
1 site
T A C G T A A T G C A T
ScaI  (4858)
1 site
A G T A C T T C A T G A
BpmI  (4448)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (4439)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (4418)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (4378)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3901)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3793)
1 site
C C C A G C G G G T C G
BseYI  (3789)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (3369)
1 site
G C