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Plasmid Files

pESC-LEU

Yeast episomal vector with a LEU2 marker, for galactose-regulated expression and tagging of up to two genes.

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pESC-LEU.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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BmgBI (7427) XbaI (7326) SnaBI (6989) NsiI (6637) ScaI (5909) BsaI (5490) BmrI (5469) AhdI (5429) AlwNI (4952) PspFI (4844) BseYI (4840) BspQI - SapI (4420) MluI (4291) BmtI (4159) NheI (4155) SacII (4153) HindIII (4140) PaeR7I - PspXI - XhoI (4133) SalI (4097) SmaI - SrfI (4092) ApaI (4091) TspMI - XmaI (4090) PspOMI (4087) BamHI (4063) BstAPI (3932) PfoI (46) PflFI - Tth111I (192) BfuAI - BspMI (759) EcoRV (1007) AflII (1438) BstEII (1718) HpaI (2174) KasI (2203) NarI (2204) SfoI (2205) PluTI (2207) DraIII (2698) NgoMIV (2799) NaeI (2801) BstZ17I (3117) PacI (3311) Eco53kI (3317) SacI (3319) BglII (3321) SpeI (3356) EagI - NotI (3363) pESC-LEU 7758 bp
BmgBI  (7427)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
XbaI  (7326)
1 site
T C T A G A A G A T C T
SnaBI  (6989)
1 site
T A C G T A A T G C A T
NsiI  (6637)
1 site
A T G C A T T A C G T A
ScaI  (5909)
1 site
A G T A C T T C A T G A
BsaI  (5490)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (5469)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (5429)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4952)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (4844)
1 site
C C C A G C G G G T C G
BseYI  (4840)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (4420)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4420)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
MluI  (4291)
1 site
A C G C G T T G C G C A
BmtI  (4159)
1 site
G C T A G C C G A T C G
NheI  (4155)
1 site
G C T A G C C G A T C G
SacII  (4153)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
HindIII  (4140)
1 site
A A G C T T T T C G A A
PaeR7I  (4133)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.