Resources
Plasmid Files

pESC-URA

Yeast episomal vector with a URA3 marker, for galactose-regulated expression and tagging of up to two genes.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pESC-URA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
Download Free Trial Get SnapGene Viewer


MfeI (6512) BmgBI (6300) XbaI (6199) SnaBI (5862) AhdI (4302) MluI (3164) PfoI (46) NdeI (330) BsgI (363) PstI - SbfI (399) BfuAI - BspMI (402) EcoRV (605) NcoI (622) BsmI (779) StuI (853) Bpu10I (1147) NgoMIV (1676) NaeI (1678) BstXI (2165) PacI (2184) Eco53kI (2190) SacI (2192) BglII (2194) BspDI - ClaI (2224) SpeI (2229) EagI - NotI (2236) EcoRI (2258) AgeI (2553) BstAPI (2805) BamHI (2936) TspMI - XmaI (2963) SmaI - SrfI (2965) SalI (2970) PaeR7I - PspXI - XhoI (3006) HindIII (3013) Acc65I (3019) KpnI (3023) SacII (3026) NheI (3028) BmtI (3032) BsrGI (3157) pESC-URA 6631 bp
MfeI  (6512)
1 site
C A A T T G G T T A A C
BmgBI  (6300)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
XbaI  (6199)
1 site
T C T A G A A G A T C T
SnaBI  (5862)
1 site
T A C G T A A T G C A T
AhdI  (4302)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
MluI  (3164)
1 site
A C G C G T T G C G C A
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
NdeI  (330)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsgI  (363)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (399)
1 site
C T G C A G G A C G T C
SbfI  (399)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (402)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (402)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
EcoRV  (605)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (622)
1 site
C C A T G G G G T A C C
BsmI  (779)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
StuI  (853)
1 site
A G G C C T T C C G G A
Bpu10I  (1147)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NgoMIV  (1676)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1678)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BstXI  (2165)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PacI  (2184)
1 site
T T A A T T A A A A T T A A T T
Eco53kI  (2190)
1 site
G A G C T C C T C G A G
SacI  (2192)
1 site
G A G C T C C T C G A G
BglII  (2194)
1 site
A G A T C T T C T A G A
BspDI  (2224)
1 site
A T C G A T T A G C T A
ClaI  (2224)
1 site
A T C G A T T A G C T A
SpeI  (2229)
1 site
A C T A G T T G A T C A
EagI  (2236)
1 site
C G G C C G G C C G G C
NotI  (2236)
1 site
G C G G C C G C C G C C G G C G
EcoRI  (2258)
1 site
G A A T