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Plasmid Files

pPIC9

Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein.

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pPIC9.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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AatII (7848) ZraI (7846) ScaI (7406) PvuI (7296) AflIII - PciI (6033) BspQI - SapI (5917) NdeI (5856) BstZ17I (5806) PflFI - Tth111I (5780) SphI (4783) SgrAI (4627) NaeI (4620) NgoMIV (4618) BfuAI - BspMI (4483) BsrGI (4298) AleI (76) Eco53kI (206) SacI (208) PmeI (413) BlpI (588) NsiI (677) XcmI (706) BamHI (938) PsiI (1144) AvaI - BsoBI - PaeR7I - XhoI (1192) BmeT110I (1193) SnaBI (1218) EcoRI (1222) AvrII (1228) EagI - NotI (1235) AgeI (1300) BtgZI (1754) FspAI (1826) XbaI (2032) Bsu36I (2088) HpaI (2138) SalI (3177) StuI (3262) DraIII (3624) NcoI (3632) BspEI (3844) pPIC9 8023 bp
AatII  (7848)
1 site
G A C G T C C T G C A G
ZraI  (7846)
1 site
G A C G T C C T G C A G
ScaI  (7406)
1 site
A G T A C T T C A T G A
PvuI  (7296)
1 site
C G A T C G G C T A G C
AflIII  (6033)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (6033)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5917)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5917)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (5856)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (5806)
1 site
G T A T A C C A T A T G
PflFI  (5780)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5780)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
SphI  (4783)
1 site
G C A T G C C G T A C G
SgrAI  (4627)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
NaeI  (4620)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4618)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BfuAI  (4483)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4483)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsrGI  (4298)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AleI  (76)
1 site
C A C N N N N G T G G T G N N N N C A C
Eco53kI  (206)
1 site
G A G C T C C T C G A G
SacI  (208)
1 site
G A G C T C C T C G A G
PmeI  (413)
1 site
G T T T A A A C C A A A T T T G
BlpI  (588)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NsiI  (677)
1 site
A T G C A T T A C G T A
XcmI  (706)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BamHI  (938)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PsiI  (1144)
1 site
T T A T A A A A T A T T
AvaI  (1192)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1192)
1 site
C Y C G R