Resources
Plasmid Files

pPICZ A

Pichia pastoris vector for methanol-inducible intracellular expression of a protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pPICZ A Sequence and MappPICZ A.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 BglII (1) AlwNI (3016) BssS α I (2773) PciI (2600) MluI (2511) BsrGI (2504) EcoRV (2337) StuI * (2303) KflI (2295) DraIII (2252) FseI (2175) SexAI * (2062) SgrAI (2013) SmaI (1989) TspMI - XmaI (1987) AatII (1945) ZraI (1943) BssHII - MauBI (1935) MscI (1902) NcoI - StyI (1897) AleI - MslI (76) BmrI (178) PflMI (190) Eco53kI (206) SacI (208) DraI - PmeI (413) BlpI (588) NsiI (677) BstXI - XcmI (706) MfeI (864) HindIII (872) BstBI (933) EcoRI (943) BsaAI - PmlI (950) SfiI (960) BsmBI (971) Acc65I (976) KpnI (980) PaeR7I - PspXI - XhoI (981) SacII (990) NotI (993) PspOMI (1006) ApaI (1010) SalI (1051) AccI (1052) AgeI (1132) BsiWI (1264) PvuII (1298) BamHI (1413) BtgZI (1472) pPICZ A 3329 bp
BglII  (1)
1 site
A G A T C T T C T A G A
AlwNI  (3016)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (2773)
1 site
C A C G A G G T G C T C
PciI  (2600)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
MluI  (2511)
1 site
A C G C G T T G C G C A
BsrGI  (2504)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRV  (2337)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
StuI  (2303)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
KflI  (2295)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
DraIII  (2252)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
FseI  (2175)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SexAI  (2062)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SgrAI  (2013)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SmaI  (1989)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (1987)
1 site
C C C G G G G G G C C C
XmaI  (1987)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
AatII  (1945)
1 site
G A C G T C C T G C A G
ZraI  (1943)
1 site
G A C G T C C T G C A G
BssHII  (1935)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1935)
1 site
C G C G C G C G G C G C G C G C
MscI  (1902)
1 site
T G G C C A A C C G G T
NcoI  (1897)
1 site
C C A T G G G G T A C C
StyI  (1897)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AleI  (76)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (76)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmrI  (178)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
PflMI  (190)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Eco53kI  (206)
1 site
G A G C T C C T C G A G
SacI  (208)
1 site
G A G C T C C T C G A G
DraI  (413)
1 site
T T T A A A A A A T T T
PmeI  (413)
1 site
G T T T A A A C C A A A T T T G
BlpI  (588)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NsiI  (677)
1 site
A T G C A T T A C G T A
BstXI  (706)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
XcmI  (706)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
MfeI  (864)
1 site
C A A T T G G T T A A C
HindIII  (872)
1 site
A A G C T T T T C G A A
BstBI  (933)
1 site
T T C G A A A A G C T T
EcoRI  (943)
1 site
G A A T T C C T T A A G
BsaAI  (950)
1 site
Y A C G T R R T G C A Y
PmlI  (950)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
SfiI  (960)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsmBI  (971)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
Acc65I  (976)
1 site
G G T A C C C C A T G G
KpnI  (980)
1 site
G G T A C C C C A T G G
PaeR7I  (981)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (981)
1 site
V C T C G A G B B G A G C T C V
XhoI  (981)
1 site
C T C G A G G A G C T C
SacII  (990)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
NotI  (993)
1 site
G C G G C C G C C G C C G G C G
PspOMI  (1006)
1 site
G G G C C C C C C G G G
ApaI  (1010)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SalI  (1051)
1 site
G T C G A C C A G C T G
AccI  (1052)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AgeI  (1132)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsiWI  (1264)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PvuII  (1298)
1 site
C A G C T G G T C G A C
BamHI  (1413)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (1472)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AOX1 promoter
2 .. 940  =  939 bp
inducible promoter, regulated by methanol
AOX1 promoter
2 .. 940  =  939 bp
inducible promoter, regulated by methanol
ori
2661 .. 3249  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2661 .. 3249  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
TEF1 promoter
1439 .. 1825  =  387 bp
promoter for EF-1α
TEF1 promoter
1439 .. 1825  =  387 bp
promoter for EF-1α
BleoR
1899 .. 2273  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
BleoR
1899 .. 2273  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
CYC1 terminator
2339 .. 2586  =  248 bp
transcription terminator for CYC1
CYC1 terminator
2339 .. 2586  =  248 bp
transcription terminator for CYC1
AOX1 terminator
1153 .. 1399  =  247 bp
transcription terminator for AOX1
AOX1 terminator
1153 .. 1399  =  247 bp
transcription terminator for AOX1
EM7 promoter
1833 .. 1880  =  48 bp
synthetic bacterial promoter
EM7 promoter
1833 .. 1880  =  48 bp
synthetic bacterial promoter
Myc
1012 .. 1041  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
1012 .. 1041  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
6xHis
1057 .. 1074  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1057 .. 1074  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
MCS
932 .. 1011  =  80 bp
multiple cloning site
MCS
932 .. 1011  =  80 bp
multiple cloning site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter