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Plasmid Files

pRS303

Yeast integrative vector with a HIS3 marker and an MCS derived from pBLUESCRIPT.

 
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pRS303.dna
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AatII (4388) ZraI (4386) XmnI (4065) ScaI (3946) BpmI (3536) BsaI (3527) AhdI (3466) AlwNI (2989) BspQI - SapI (2457) ApaI (2173) PfoI (46) AfeI (211) BseRI (301) BsaBI * (560) MscI (710) BsmI (715) BbsI (789) BsiWI (915) NheI (1007) BmtI (1011) BclI * (1051) NsiI (1282) BssHII (1311) PsiI (1519) DraIII (1647) BtgZI (1648) NgoMIV (1748) NaeI (1750) Eco53kI (2075) SacI (2077) BtgI (2081) SacII (2084) EagI - NotI (2090) XbaI (2097) SpeI (2103) BamHI (2109) TspMI - XmaI (2115) SmaI (2117) EcoRI (2127) EcoRV (2135) BspDI - ClaI (2146) SalI (2154) HincII (2156) AbsI - PaeR7I - PspXI - XhoI (2160) PspOMI (2169) pRS303 4443 bp
AatII  (4388)
1 site
G A C G T C C T G C A G
ZraI  (4386)
1 site
G A C G T C C T G C A G
XmnI  (4065)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3946)
1 site
A G T A C T T C A T G A
BpmI  (3536)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (3527)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3466)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2989)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspQI  (2457)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2457)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
ApaI  (2173)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
AfeI  (211)
1 site
A G C G C T T C G C G A
BseRI  (301)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsaBI  (560)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MscI  (710)
1 site
T G G C C A A C C G G T
BsmI  (715)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BbsI  (789)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsiWI  (915)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NheI  (1007)
1 site
G C T A G C C G A T C G
BmtI  (1011)
1 site
G C T A G C C G A T C G
BclI  (1051)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NsiI  (1282)
1 site
A T G C A T T A C G T A
BssHII  (1311)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PsiI  (1519)
1 site
T T A T A A A A T A T T
DraIII  (1647)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BtgZI  (1648)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
NgoMIV  (1748)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1750)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
Eco53kI  (2075)
1 site
G A G C T C C T C G A G
SacI  (2077)
1 site
G A G C T C C T C G A G
BtgI  (2081)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (2084)
1 site