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Plasmid Files

pRS415

Yeast centromere vector with a LEU2 marker and an MCS derived from pBLUESCRIPT II.

 
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pRS415.dna
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SwaI (5477) PmlI (5453) ScaI (5000) BsaI (4581) AhdI (4520) AlwNI (4043) PspFI (3935) BseYI (3931) SacI (3233) Eco53kI (3231) SacII (3224) AleI (3223) EagI - NotI (3212) XbaI (3205) SpeI (3199) BamHI (3193) SmaI (3189) TspMI - XmaI (3187) PstI (3185) HindIII (3163) SalI (3148) AbsI - PaeR7I - PspXI - XhoI (3142) ApaI (3137) PspOMI (3133) PfoI (46) PflFI - Tth111I (192) BsrGI (632) BfuAI - BspMI (759) XcmI (1201) AflII (1438) AgeI (1503) BstEII (1718) HpaI (2174) KasI (2203) NarI (2204) SfoI (2205) PluTI (2207) DraIII (2698) BtgZI (2699) NgoMIV (2799) NaeI (2801) pRS415 6021 bp
SwaI  (5477)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PmlI  (5453)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
ScaI  (5000)
1 site
A G T A C T T C A T G A
BsaI  (4581)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4520)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4043)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3935)
1 site
C C C A G C G G G T C G
BseYI  (3931)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
SacI  (3233)
1 site
G A G C T C C T C G A G
Eco53kI  (3231)
1 site
G A G C T C C T C G A G
SacII  (3224)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (3223)
1 site
C A C N N N N G T G G T G N N N N C A C
EagI  (3212)
1 site
C G G C C G G C C G G C
NotI  (3212)
1 site
G C G G C C G C C G C C G G C G
XbaI  (3205)
1 site
T C T A G A A G A T C T
SpeI  (3199)
1 site
A C T A G T T G A T C A
BamHI  (3193)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (3189)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (3187)
1 site
C C C G G G G G G C C C
XmaI  (3187)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
PstI  (3185)
1 site
C T G C A G G A C G T C
HindIII  (3163)
1 site
A A G C T T T T C G A A
SalI  (3148)
1 site
G T C G A C C A G C T G
AbsI  (3142)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (3142)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3142)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3142)
1 site
C T C G A G G A G C T C
ApaI  (3137)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3133)
1 site
G G G C C C C C C G G G
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (192)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrGI  (632)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BfuAI  (759)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (759)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
XcmI  (1201)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
AflII  (1438)
1 site
C T T A A G G A A T T C <