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pYC2/NT B

Centromeric vector for galactose-inducible expression of N-terminally tagged proteins in S. cerevisiae. For other reading frames, use pYC2/NT A or pYC2/NT C.

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pYC2 NT B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SpeI (4698) NaeI (4558) MreI - NgoMIV - SgrAI (4556) SwaI (4075) BsaAI - PmlI (4051) BspDI - ClaI (4031) NdeI (3882) BsgI (3853) SbfI (3819) BfuAI - BspMI (3808) XcmI (3670) BsmI (3437) StuI (3361) BsmBI (3171) Bpu10I (3064) NsiI (2974) BsaHI (2649) PvuI (2482) BtgZI (297) BstAPI (323) PvuII (471) SspI (497) HindIII (501) Acc65I (602) KpnI (606) Bsu36I (607) BamHI (611) EcoRI (626) EagI - NotI (653) AvaI - BsoBI - PaeR7I - PspXI - XhoI (659) BmeT110I (660) XbaI (665) PmeI (758) BsrGI (950) DrdI (1327) BciVI (1422) BspHI (1939) AhdI (2112) AfeI (2142) BglI (2232) FspI (2334) pYC2/NT B 4703 bp
SpeI  (4698)
1 site
A C T A G T T G A T C A
NaeI  (4558)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
MreI  (4556)
1 site
C G C C G G C G G C G G C C G C
NgoMIV  (4556)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SgrAI  (4556)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SwaI  (4075)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BsaAI  (4051)
1 site
Y A C G T R R T G C A Y
PmlI  (4051)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BspDI  (4031)
1 site
A T C G A T T A G C T A
ClaI  (4031)
1 site
A T C G A T T A G C T A
NdeI  (3882)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsgI  (3853)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SbfI  (3819)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (3808)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N )