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Simulate a Restriction Digest

How do I simulate an agarose gel for a restriction digest?

Specify the Sequence for Digestion

To specify the sequence, expand the Lane [N] menu, then choose the desired file.

Choose Restriction Digestion

To specify a restriction digest, choose cut with (default) from the expanded menu.

Specify the Digestion Enzymes

To specify the digestion enzymes, type the enzyme name in the menu box to auto-search the menu. Tab to specify other digestion enzymes. Alternatively, choose the enzyme name from the expanded menu (type the name to auto-search within the expanded menu).

The resulting digest simulation will appear.

Apply to All Lanes

If you wish to simulate the digestion of multiple sequences with the same enzyme(s), click the Apply to All Lanes button.

View the Simulated Agarose Gel

The simulated agarose gel for the restriction digest will be shown.

Rapidly Simulate a Restriction Digest

To simulate a restriction digest from a sequence window, click one or more enzymes, then click Tools → Simulate Agarose Gel.

The resulting digest will appear in the next available lane in the Agarose Gel Simulation dialog.

Repeat this step to populate other lanes.

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